一个棘手的难题：通过基因编码蛋白传感器对疟原虫中的血红素进行细胞内检测和定量分析
A Tough Nut to Crack: Intracellular Detection and Quantification of Heme in Malaria Parasites by a Genetically Encoded Protein Sensor.
作者信息
Wißbrock Amelie, Imhof Diana
机构信息
University of Bonn, Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, Brühler Strasse 7, 53119, Bonn, Germany.
出版信息
Chembiochem. 2017 Aug 17;18(16):1561-1564. doi: 10.1002/cbic.201700274. Epub 2017 Jul 5.
How free is heme? A fluorescent sensor has been used by Niles and co-workers to detect heme in P. falciparum. ECFP and EYFP were attached to its termini to give a FRET signal that was quenched by heme binding. The sensor was used to investigate alterations in the labile heme pool during the parasite's life cycle as well as under the influence of the drug chloroquine.
血红素的游离程度如何？奈尔斯及其同事使用一种荧光传感器来检测恶性疟原虫中的血红素。增强型青色荧光蛋白（ECFP）和增强型黄色荧光蛋白（EYFP）连接在其末端，以产生一个因血红素结合而淬灭的荧光共振能量转移（FRET）信号。该传感器用于研究寄生虫生命周期中以及在药物氯喹影响下不稳定血红素池的变化。
Zotero 插件