Xu Qianqian, Sun Junfeng, Gao Mengying, Zhao Shasha, Liu Huairan, Zhang TingTing, Han Zongxi, Kong Xiangang, Liu Shengwang
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
J Vet Diagn Invest. 2017 Jul;29(4):489-498. doi: 10.1177/1040638717709855. Epub 2017 May 26.
Four Newcastle disease virus (NDV) strains were isolated from domestic, commercial geese that showed clinical signs that were believed to be the result of NDV infections. The genetic, antigenic, and pathogenic characteristics of the 4 NDVs were compared with those of NDV strains that were isolated from chickens. The complete genomes of 2 of the NDV strains contained 15,186 nucleotides (nt); the other 2 contained 15,192 nt, and exhibited the typical genomic organization of genotype II NDV and molecular characteristics of VIId NDVs. Phylogenetic analysis confirmed that the genotype II and VIId NDVs that were isolated from geese belonged to the same clusters as the corresponding genotypes of the chicken isolates. A serologic assay demonstrated that the antigenic relatedness among the NDVs was associated with their genotypes, rather than their hosts, and that amino acid substitutions in the F and/or HN proteins may contribute to the antigenic differences among these NDV genotypes. Geese infected with genotype VIId NDVs that were isolated from geese and chickens showed similar pathologic characteristics. NDVs that were isolated from geese did not differ in genetic, serologic, and pathogenic characteristics from those isolated from chickens, indicating that these NDVs were derived from chicken NDVs. Given the significance of geese in NDV epidemiology, effective biosecurity measures should be adopted to prevent the interspecies transmission of NDVs.
从出现被认为是新城疫病毒（NDV）感染所致临床症状的家养商业鹅中分离出4株新城疫病毒。将这4株NDV的基因、抗原和致病特性与从鸡中分离出的NDV毒株进行了比较。其中2株NDV毒株的完整基因组含有15,186个核苷酸（nt）；另外2株含有15,192 nt，呈现出典型的II型NDV基因组结构和VII d型NDV的分子特征。系统发育分析证实，从鹅中分离出的II型和VII d型NDV与鸡分离株的相应基因型属于同一簇。血清学检测表明，NDV之间的抗原相关性与其基因型有关，而非宿主，并且F和/或HN蛋白中的氨基酸替换可能导致这些NDV基因型之间的抗原差异。感染从鹅和鸡中分离出的VII d型NDV的鹅表现出相似的病理特征。从鹅中分离出的NDV在基因、血清学和致病特性方面与从鸡中分离出的NDV没有差异，表明这些NDV源自鸡的NDV。鉴于鹅在NDV流行病学中的重要性，应采取有效的生物安全措施以防止NDV的种间传播。
