State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, People's Republic of China.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
Antonie Van Leeuwenhoek. 2017 Sep;110(9):1179-1187. doi: 10.1007/s10482-017-0890-y. Epub 2017 May 26.
A novel strain, 1433, was isolated from leaves of Chinese red pepper (Huajiao, Zanthoxylum bungeanum Maxim) collected from Gansu province in northwestern China, and was characterised by a polyphasic approach. Cells of strain 1433 were observed to be Gram-stain positive, aerobic, asporogenous, rod shaped, motile and to have peritrichous flagella. The strain was observed to grow at a range of temperatures and pH, 4-45 °C (optimum 28-32 °C) and 6.0-10.0 (optimum pH 6.0-7.0), respectively. Growth was found to occur in the presence of 0-7% (w/v) NaCl [optimum 0-3% (w/v)]. The G+C content of the genomic DNA was determined to be 41.9 mol% and the cell wall peptidoglycan found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7 and the major polar lipids as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified polar lipid and three unidentified phospholipids. The major cellular fatty acids were identified as iso-C (31.6%), anteiso-C (26.9%) and iso-C (17.1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1433 is a member of the genus Bacillus and is closely related to Bacillus aryabhattai DSM 21047 (99.4% sequence similarity) and Bacillus megaterium DSM 32 (99.2%). DNA-DNA relatedness of the novel strain 1433 with B. aryabhattai DSM 21047 and B. megaterium DSM 32 was 33.8 ± 2.8% and 28.9 ± 3.4%, respectively. On the basis of the polyphasic evidence presented, strain 1433 is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus zanthoxyli sp. nov. The type strain is 1433 (= CCTCC AB 2016326 = KCTC33730).
一株新的菌株 1433 是从中国甘肃省采集的中国红辣椒（花椒，Zanthoxylum bungeanum Maxim）叶片中分离出来的，通过多相方法进行了特征描述。观察到菌株 1433 的细胞革兰氏染色阳性，需氧，无孢子形成，杆状，运动，周生鞭毛。该菌株在温度和 pH 值范围为 4-45°C（最佳温度为 28-32°C）和 6.0-10.0（最佳 pH 值为 6.0-7.0）时均可生长。在含有 0-7%（w/v）NaCl 的情况下发现生长[最佳为 0-3%（w/v）]。基因组 DNA 的 G+C 含量为 41.9 mol%，细胞壁肽聚糖含有 meso-二氨基庚二酸。主要的menaquinone 被鉴定为 MK-7，主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的极性脂和三种未鉴定的磷脂。主要的细胞脂肪酸被鉴定为 iso-C（31.6%）、anteiso-C（26.9%）和 iso-C（17.1%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 1433 是芽孢杆菌属的一个成员，与 Bacillus aryabhattai DSM 21047（99.4%序列相似性）和 Bacillus megaterium DSM 32（99.2%）密切相关。新型菌株 1433 与 Bacillus aryabhattai DSM 21047 和 Bacillus megaterium DSM 32 的 DNA-DNA 同源性分别为 33.8±2.8%和 28.9±3.4%。基于提供的多相证据，菌株 1433 被认为代表芽孢杆菌属的一个新种，我们建议将其命名为 Bacillus zanthoxyli sp. nov. 。模式菌株为 1433（=CCTCC AB 2016326=KCTC 33730）。
