College of Environment, Shenyang University, Shenyang, China.
College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
PLoS One. 2020 Aug 6;15(8):e0237194. doi: 10.1371/journal.pone.0237194. eCollection 2020.
We aimed to profile the metabolism of soybean roots that were infected with soybean cyst nematodes and treated with Bacillus simplex to identify metabolic differences that may explain nematode resistance. Compared with control soybean roots, B. simplex-treated soybean roots contained lower levels of glucose, fructose, sucrose, and trehalose, which reduced the nematodes' food source. Furthermore, treatment with B. simplex led to higher levels of melibiose, gluconic acid, lactic acid, phytosphingosine, and noradrenaline in soybean roots, which promoted nematocidal activity. The levels of oxoproline, maltose, and galactose were lowered after B. simplex treatment, which improved disease resistance. Collectively, this study provides insight into the metabolic alterations induced by B. simplex treatment, which affects the interactions with soybean cyst nematodes.
本研究旨在分析感染大豆胞囊线虫的大豆根系和经枯草芽孢杆菌处理的大豆根系的代谢组，以鉴定可能解释线虫抗性的代谢差异。与对照大豆根系相比，枯草芽孢杆菌处理的大豆根系中的葡萄糖、果糖、蔗糖和海藻糖含量较低，这降低了线虫的食物来源。此外，枯草芽孢杆菌处理还导致大豆根系中的蜜二糖、葡萄糖酸、乳酸、植物鞘氨醇和去甲肾上腺素的含量升高，从而促进了杀线虫活性。枯草芽孢杆菌处理后，大豆根系中的羟脯氨酸、麦芽糖和半乳糖的含量降低，从而提高了抗病性。总之，本研究深入了解了枯草芽孢杆菌处理诱导的代谢变化，这些变化影响了与大豆胞囊线虫的相互作用。
