Department of Dermatology and Dermatologic Surgery, Medical University of South Carolina, Charleston, South Carolina.
Department of Dermatology and Dermatologic Surgery, Medical University of South Carolina, Charleston, South Carolina.
J Am Acad Dermatol. 2017 Aug;77(2):268-273.e6. doi: 10.1016/j.jaad.2017.03.016. Epub 2017 May 24.
Hidradenitis suppurativa (HS) is a debilitating skin disease characterized by painful recurrent nodules and abscesses caused by chronic inflammation. Early events in the development of HS are believed to occur in the folliculopilosebaceous unit; however, the signaling pathways behind this mechanism are unknown. Sphingolipids, such as ceramide, are essential components of the skin and appendages and have important structural and signaling roles.
We sought to explore whether the gene expression of enzymes involved in sphingolipid metabolic pathways is altered in HS.
A microarray data set including 30 samples was used to compare the expression of sphingolipid-related enzymes in inflammatory skin lesions from HS patients (n = 17) with the expression in clinically healthy skin tissue (n = 13). Differential expression of sphingolipid metabolism-related genes was analyzed using Gene Expression Omnibus 2R.
HS lesional skin samples have significantly decreased expression of enzymes generating ceramide and sphingomyelin, increased expression of enzymes catabolizing ceramide to sphingosine, and increased expression of enzymes converting ceramide to galactosylceramide and gangliosides.
Limitations of this study include assessing the expression of sphingolipid-related enzymes without assessing the levels of the related sphingolipids.
Our study suggests that sphingolipid metabolism is altered in HS lesional skin compared with normal skin.
化脓性汗腺炎（HS）是一种使人衰弱的皮肤病，其特征为慢性炎症引起的疼痛性复发性结节和脓肿。HS 发病的早期事件被认为发生在毛囊皮脂腺单位中；然而，该机制背后的信号通路尚不清楚。神经酰胺等鞘脂类是皮肤和附属物的重要组成部分，具有重要的结构和信号作用。
我们试图探讨鞘脂代谢途径相关酶的基因表达是否在 HS 中发生改变。
使用包含 30 个样本的微阵列数据集，比较 HS 患者（n=17）炎症性皮肤损伤中鞘脂相关酶的表达与临床健康皮肤组织（n=13）中的表达。使用基因表达综合数据库 2R 分析鞘脂代谢相关基因的差异表达。
HS 皮损皮肤样本中产生神经酰胺和神经鞘磷脂的酶表达显著降低，分解神经酰胺为神经鞘氨醇的酶表达增加，以及将神经酰胺转化为半乳糖脑苷脂和神经节苷脂的酶表达增加。
本研究的局限性包括评估鞘脂相关酶的表达而不评估相关鞘脂的水平。
与正常皮肤相比，HS 皮损皮肤中的鞘脂代谢发生改变。
