International School for Advanced Studies (SISSA), Trieste, Italy.
Life Science Department, University of Trieste, Trieste, Italy; ELETTRA Synchrotron Light Source, Trieste, Italy.
Nanomedicine. 2018 Oct;14(7):2521-2532. doi: 10.1016/j.nano.2017.01.020. Epub 2017 May 25.
Carbon nanotube-based biomaterials critically contribute to the design of many prosthetic devices, with a particular impact in the development of bioelectronics components for novel neural interfaces. These nanomaterials combine excellent physical and chemical properties with peculiar nanostructured topography, thought to be crucial to their integration with neural tissue as long-term implants. The junction between carbon nanotubes and neural tissue can be particularly worthy of scientific attention and has been reported to significantly impact synapse construction in cultured neuronal networks. In this framework, the interaction of 2D carbon nanotube platforms with biological membranes is of paramount importance. Here we study carbon nanotube ability to interfere with lipid membrane structure and dynamics in cultured hippocampal neurons. While excluding that carbon nanotubes alter the homeostasis of neuronal membrane lipids, in particular cholesterol, we document in aged cultures an unprecedented functional integration between carbon nanotubes and the physiological maturation of the synaptic circuits.
基于碳纳米管的生物材料在许多义肢设备的设计中发挥了关键作用，特别是在新型神经界面的生物电子元件的开发中。这些纳米材料结合了优异的物理和化学性质以及独特的纳米结构形貌，被认为对其作为长期植入物与神经组织的整合至关重要。碳纳米管和神经组织之间的连接特别值得引起科学界的关注，并且已经有报道表明，它会显著影响培养神经元网络中的突触形成。在这个框架中，二维碳纳米管平台与生物膜的相互作用至关重要。在这里，我们研究了碳纳米管干扰培养海马神经元中脂质膜结构和动力学的能力。虽然碳纳米管不会改变神经元膜脂质的内环境稳态，特别是胆固醇，但我们在老化培养物中记录到了碳纳米管与突触回路的生理成熟之间前所未有的功能整合。
