Szewczenko Janusz, Kajzer Wojciech, Grygiel-Pradelok Magdalena, Jaworska Joanna, Jelonek Katarzyna, Nowińska Katarzyna, Gawliczek Maria, Libera Marcin, Marcinkowski Andrzej, Kasperczyk Janusz
Department of Biomechatronics, Faculty of Biomedical Engineering, Silesian University of Technology, Zabrze, Poland.
Department of Biomaterials and Medical Devices Engineering, Faculty of Biomedical Engineering, Silesian University of Technology, Zabrze, Poland.
Acta Bioeng Biomech. 2017;19(1):173-179.
The aim of the study was to determine the influence of PLGA bioresorbable polymer coating on corrosion resistance of metal biomaterial. Polymer coating deposited by immersion method was applied. Corrosion resistance of metal biomaterials (stainless steel, Ti6Al4V, Ti6Al7Nb) coated with PLGA polymer, after 90 days exposure to Ringer's solution was tested. The amount of metal ions released to the solution was also investigated (inductively coupled plasma-atomic emission spectrometry (ICP-AES) method). The surface of the samples was observed using atomic force microscopy (AFM) and scanning electron microscopy (SEM). Degradation of PLGA was monitored with the use of the 1H NMR spectroscopy and GPC (Gel Permeation Chromatography). The studies were carried out for non-sterilized (NS) and sterilized (S) samples. Application of the polymer coating causes a reduction of release of metal ions to the solution. Depending on metal substrate different course of destruction of polymer layer was observed. After 90 days of incubation in Ringer's solution polymer layer was highly degraded, however, the composition of copolymer (ratio of the comonomeric units in the chain) remained unchanged during the whole process, which suggests even degradation. The polymer layer reduced degradation kinetics of the metal substrate. Moreover, degradation process did not change surface morphology of metal substrate and did not disturb its integrity. The results obtained indicate that the applied polymer layer improves corrosion resistance of the alloys being investigated. Thus, the developed implants with bioresorbable coatings could be advantageous for medical applications.
本研究的目的是确定聚乳酸-羟基乙酸共聚物（PLGA）生物可吸收聚合物涂层对金属生物材料耐腐蚀性的影响。采用浸涂法施加聚合物涂层。对涂覆有PLGA聚合物的金属生物材料（不锈钢、Ti6Al4V、Ti6Al7Nb）在暴露于林格氏溶液90天后的耐腐蚀性进行了测试。还研究了释放到溶液中的金属离子量（电感耦合等离子体原子发射光谱法（ICP-AES））。使用原子力显微镜（AFM）和扫描电子显微镜（SEM）观察样品表面。利用1H NMR光谱和凝胶渗透色谱法（GPC）监测PLGA的降解情况。对未灭菌（NS）和灭菌（S）样品进行了研究。聚合物涂层的应用导致金属离子向溶液中的释放减少。根据金属基材的不同，观察到聚合物层的破坏过程也不同。在林格氏溶液中孵育90天后，聚合物层高度降解，然而，共聚物的组成（链中共聚单体单元的比例）在整个过程中保持不变，这表明降解均匀。聚合物层降低了金属基材的降解动力学。此外，降解过程没有改变金属基材的表面形态，也没有破坏其完整性。所得结果表明，所施加的聚合物层提高了所研究合金的耐腐蚀性。因此，所开发的具有生物可吸收涂层的植入物在医学应用中可能具有优势。
