Nocchetti Morena, Piccinini Michela, Pietrella Donatella, Antognelli Cinzia, Ricci Maurizio, Di Michele Alessandro, Jalaoui Layla, Ambrogi Valeria
Department of Pharmaceutical Science, University of Perugia, 06123 Perugia, Italy.
Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy.
J Funct Biomater. 2025 Jan 12;16(1):22. doi: 10.3390/jfb16010022.
Magnesium alloys are promising biomaterials to be used as temporary implants due to their biocompatibility and biodegradability. The main limitation in the use of these alloys is their rapid biodegradation. Moreover, the risk of microbial infections, often following the implant surgery and hard to eradicate, is another challenge. Thus, with the aim of reducing biodegradability and conferring antibiofilm activity, sheets of the magnesium alloy AZ31 were properly modified with the introduction of hydroxy (polyethyleneoxy)propyl silane (PEG) and quaternary ammonium silane chains (QAS). The derivatized sheets were characterized by ATR-FTIR spectroscopy and their performances as concerns their stability, Mg in vitro release, and in vitro bioactivity were evaluated as well. The results showed an increased stability with a reduction in corrosion, a slower Mg ion release, and the formation of hydroxyapatite in the sheets' surface. In addition, cytotoxicity evaluations were carried out on human gingival fibroblasts showing that the AZ31 and AZ31-PEG plates had good cytocompatibility. Finally, the antibiofilm activity on , and was carried out by evaluating the capacity of inhibition of biofilm adhesion and formation. The results demonstrated a significant reduction in biofilm formation by on AZ31-QAS.
镁合金因其生物相容性和生物可降解性而有望成为用作临时植入物的生物材料。使用这些合金的主要限制在于它们的快速生物降解性。此外，植入手术后常出现且难以根除的微生物感染风险是另一个挑战。因此，为了降低生物降解性并赋予抗生物膜活性，通过引入羟基（聚乙氧基）丙基硅烷（PEG）和季铵硅烷链（QAS）对镁合金AZ31板材进行了适当改性。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）对衍生化板材进行了表征，并评估了它们在稳定性、镁的体外释放以及体外生物活性方面的性能。结果表明稳定性有所提高，腐蚀减少，镁离子释放变慢，且板材表面形成了羟基磷灰石。此外，对人牙龈成纤维细胞进行了细胞毒性评估，结果表明AZ31和AZ31-PEG平板具有良好的细胞相容性。最后，通过评估抑制生物膜粘附和形成的能力，对金黄色葡萄球菌、大肠杆菌和白色念珠菌进行了抗生物膜活性测试。结果表明，AZ31-QAS对金黄色葡萄球菌生物膜形成有显著减少。
