Jin Peng, Gu Wenzhu, Lai Yayu, Zheng Wei, Zhou Qi, Wu Xiaojing
Cardiovascular Department of Xinqiao Hospital, Third Military Medical University, Chongqing, China.
Cardiovascular Department of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Cell Physiol Biochem. 2017;41(6):2150-2160. doi: 10.1159/000475569. Epub 2017 Apr 20.
MicroRNA-206 (miR-206), a muscle-specific miRNA, regulates the growth of cardiac myocytes and pulmonary artery smooth muscle cells. However, it remains unknown whether miR-206 is involved in pulmonary hypertension (PH) due to left heart diseases (PH-LHD). This study was designed to investigate the correlation between miR-206 and PH in patients with LHD.
In 82 consecutively enrolled LHD patients, we examined the serum levels of miR-206 and analyzed its correlations with pulmonary artery systolic pressure (PASP) and cardiac function. Another 36 age- and sex-matched subjects served as healthy controls.
The patients were divided into the LHD group (n=47, PASP<50 mmHg) and the PH-LHD group (n=35, PASP≥50 mmHg). The level of miR-206 was significantly decreased in the PH-LHD group compared with that of the LHD and healthy control groups. In addition, the miR-206 level was correlated with PASP (r=-0.305, p<0.001) but not with systemic blood pressure. Univariate analyses showed that miR-206, brain natriuretic peptide (BNP), left ventricular ejection fraction and left atrial longitudinal diameter (LAD) were significantly related to PASP. Multivariate regression analysis identified miR-206 as an independent predictive factor for PH. MiR-206 alone (cut-off <0.66) demonstrated a sensitivity of 68.60% and a specificity of 65.80% in predicting PH. Moreover, the combination of miR-206, BNP and LAD (cut-off 0.21) showed a sensitivity of 97.10% and a specificity of 80.30% in predicting PH in LHD patients.
A decreased circulating miR-206 level was associated with increased PASP in LHD patients. Thus, the level of miR-206, especially combined with BNP and LAD, might be helpful in the detection of PH in LHD patients.
微小RNA-206（miR-206）是一种肌肉特异性微小RNA，可调节心肌细胞和肺动脉平滑肌细胞的生长。然而，miR-206是否参与左心疾病所致肺动脉高压（PH-LHD）仍不清楚。本研究旨在探讨LHD患者中miR-206与PH的相关性。
在连续纳入的82例LHD患者中，我们检测了血清miR-206水平，并分析其与肺动脉收缩压（PASP）和心功能的相关性。另外36例年龄和性别匹配的受试者作为健康对照。
患者分为LHD组（n = 47，PASP < 50 mmHg）和PH-LHD组（n = 35，PASP≥50 mmHg）。与LHD组和健康对照组相比，PH-LHD组的miR-206水平显著降低。此外，miR-206水平与PASP相关（r = -0.305，p < 0.001），但与体循环血压无关。单因素分析显示，miR-206、脑钠肽（BNP）、左心室射血分数和左心房内径（LAD）与PASP显著相关。多因素回归分析确定miR-206是PH的独立预测因素。单独的miR-206（临界值<0.66）在预测PH时的敏感性为68.60%，特异性为65.80%。此外，miR-206、BNP和LAD的联合检测（临界值0.21）在预测LHD患者的PH时敏感性为97.10%，特异性为80.30%。
LHD患者循环miR-206水平降低与PASP升高有关。因此，miR-206水平，尤其是与BNP和LAD联合检测，可能有助于LHD患者中PH的检测。
