Pitchaimani Arunkumar, Nguyen Tuyen Duong Thanh, Koirala Mukund, Zhang Yuntao, Aryal Santosh
Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA; Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State University, Manhattan, KS 66506, USA; Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.
Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA; Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State University, Manhattan, KS 66506, USA.
Toxicol In Vitro. 2017 Sep;43:29-39. doi: 10.1016/j.tiv.2017.05.020. Epub 2017 May 26.
In vitro cell-nanoparticle (NP) studies involve exposure of NPs onto the monolayer cells growing at the bottom of a culture plate, and assumed that the NPs evenly distributed for a dose-responsive effect. However, only a few proportion of the administered dose reaches the cells depending on their size, shape, surface, and density. Often the amount incubated (administered dose) is misled as a responsive dose. Herein, we proposed a cell adhesion-migration (CAM) strategy, where cells incubated with the NP coated cell culture substrate to maximize the cell-NP interaction and investigated the physiological properties of the cells. In the present study, cell adhesion and migration pattern of human breast cancer cell (MCF-7) and mouse melanoma cell (B16-F10) on cell culture substrate decorated with toxic (cetyltrimethylammonium bromide, CTAB) and biocompatible (poly (sodium 4-styrenesulphonate), PSS) gold nanoparticles (AuNPs) of different sizes (5 and 40nm) were investigated and evaluated for cellular uptake efficiency, proliferation, and toxicity. Results showed enhanced cell adhesion, migration, and nanoparticle uptake only on biocompatible PSS coated AuNP, irrespective of its size. Whereas, cytotoxic NP shows retard proliferation with reduced cellular uptake efficiency. Considering the importance of cell adhesion and migration on cellular uptake and cytotoxicity assessment of nanoparticle, CAM strategy would hold great promises in cell-NP interaction studies.
体外细胞-纳米颗粒（NP）研究涉及将纳米颗粒暴露于培养板底部生长的单层细胞上，并假定纳米颗粒均匀分布以产生剂量响应效应。然而，根据纳米颗粒的大小、形状、表面和密度，只有一小部分给药剂量能够到达细胞。通常，孵育的量（给药剂量）被误当作响应剂量。在此，我们提出了一种细胞黏附-迁移（CAM）策略，即让细胞与包被有纳米颗粒的细胞培养底物孵育，以最大化细胞与纳米颗粒的相互作用，并研究细胞的生理特性。在本研究中，研究并评估了人乳腺癌细胞（MCF-7）和小鼠黑色素瘤细胞（B16-F10）在装饰有不同尺寸（5纳米和40纳米）的毒性（十六烷基三甲基溴化铵，CTAB）和生物相容性（聚（4-苯乙烯磺酸钠），PSS）金纳米颗粒（AuNPs）的细胞培养底物上的细胞黏附和迁移模式，以及细胞摄取效率、增殖和毒性。结果表明，无论尺寸大小，仅在生物相容性PSS包被的AuNP上细胞黏附、迁移和纳米颗粒摄取增强。而具有细胞毒性的纳米颗粒则显示增殖受抑制且细胞摄取效率降低。考虑到细胞黏附和迁移对纳米颗粒细胞摄取及细胞毒性评估的重要性，CAM策略在细胞-纳米颗粒相互作用研究中具有广阔前景。
