Chen Zhitong, Simonyan Hayk, Cheng Xiaoqian, Gjika Eda, Lin Li, Canady Jerome, Sherman Jonathan H, Young Colin, Keidar Michael
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052, USA.
Department of Pharmacology and Physiology, The George Washington University, Washington, DC 20052, USA.
Cancers (Basel). 2017 May 30;9(6):61. doi: 10.3390/cancers9060061.
Cold atmospheric plasma (CAP) treatment is a rapidly expanding and emerging technology for cancer treatment. Direct CAP jet irradiation is limited to the skin and it can also be invoked as a supplement therapy during surgery as it only causes cell death in the upper three to five cell layers. However, the current cannulas from which the plasma emanates are too large for intracranial applications. To enhance efficiency and expand the applicability of the CAP method for brain tumors and reduce the gas flow rate and size of the plasma jet, a novel micro-sized CAP device (µCAP) was developed and employed to target glioblastoma tumors in the murine brain. Various plasma diagnostic techniques were applied to evaluate the physics of helium µCAP such as electron density, discharge voltage, and optical emission spectroscopy (OES). The direct and indirect effects of µCAP on glioblastoma (U87MG-RedFluc) cancer cells were investigated in vitro The results indicate that µCAP generates short- and long-lived species and radicals (i.e., hydroxyl radical (OH), hydrogen peroxide (H₂O₂), and nitrite (NO₂), etc.) with increasing tumor cell death in a dose-dependent manner. Translation of these findings to an in vivo setting demonstrates that intracranial µCAP is effective at preventing glioblastoma tumor growth in the mouse brain. The µCAP device can be safely used in mice, resulting in suppression of tumor growth. These initial observations establish the µCAP device as a potentially useful ablative therapy tool in the treatment of glioblastoma.
冷大气等离子体（CAP）治疗是一种用于癌症治疗的迅速发展的新兴技术。直接的CAP射流辐照仅限于皮肤，并且由于它仅导致最上面的三到五层细胞死亡，所以也可在手术期间用作辅助治疗。然而，目前产生等离子体的插管对于颅内应用来说太大了。为了提高效率并扩大CAP方法对脑肿瘤的适用性，同时降低等离子体射流的气体流速和尺寸，开发了一种新型的微型CAP设备（µCAP），并将其用于靶向小鼠脑中的胶质母细胞瘤。应用各种等离子体诊断技术来评估氦气µCAP的物理特性，如电子密度、放电电压和光发射光谱（OES）。在体外研究了µCAP对胶质母细胞瘤（U87MG-RedFluc）癌细胞的直接和间接作用。结果表明，µCAP产生短寿命和长寿命的物质及自由基（即羟基自由基（OH）、过氧化氢（H₂O₂）和亚硝酸盐（NO₂）等），并以剂量依赖的方式增加肿瘤细胞死亡。将这些发现转化到体内环境表明，颅内µCAP在预防小鼠脑中胶质母细胞瘤的生长方面是有效的。µCAP设备可以在小鼠中安全使用，从而抑制肿瘤生长。这些初步观察结果确立了µCAP设备作为治疗胶质母细胞瘤的一种潜在有用的消融治疗工具。
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