Peeters Sophie, Wu Peter B, Haist Blake, Armellini Amber, Kim Wi Jin, Chen Zhitong, Obenchain Richard, Ayad George, Ge Weihong, Bhaduri Aparna, Sabiston Graeme, Prins Robert M, Wirz Richard, Wang Anthony C
Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Los Angeles, CA, USA.
Sci Rep. 2025 Jul 1;15(1):20838. doi: 10.1038/s41598-025-05916-y.
Chordomas remain one of the most difficult-to-treat of skull base tumors. The best chance of survival and cure to date is with en bloc radical surgical resection, followed by adjuvant radiotherapy. Oftentimes, tumor infiltration into functionally critical, or difficult-to-access areas, precludes optimal resection. The median overall survival for these tumors is 116 months. Cold atmospheric plasma (CAP) is generated by applying a high voltage electric field to helium or argon feed gases, resulting in reactive atmospheric species. Over the past decade, CAP has been applied experimentally in a number of oncologic conditions and has demonstrated anti-tumor effects both in vitro and in vivo. Additionally, CAP has been shown to increase cancer cells' sensitivity to radiation and could therefore be a useful tool in improving chordoma recurrence rates as an intraoperative adjuvant therapy to the current standard of care. To date, there are no studies in the literature examining the efficacy of CAP in inducing cytotoxicity in chordoma. We treated CH2, CH7 and UM-Chor1 chordoma cells with CAP, measuring resulting cell viability and intracellular ROS accumulation. Here, we show a dose-dependent increase in intracellular ROS and cell death with direct CAP exposure in vitro, finding an exquisite sensitivity of chordoma cells to CAP-mediated cytotoxicity.
脊索瘤仍然是最难治疗的颅底肿瘤之一。迄今为止，实现生存和治愈的最佳机会是进行整块根治性手术切除，然后进行辅助放疗。通常情况下，肿瘤浸润到功能关键或难以触及的区域，会妨碍进行最佳切除。这些肿瘤的中位总生存期为116个月。冷大气等离子体（CAP）是通过向氦气或氩气进料气体施加高压电场产生的，会产生活性大气物质。在过去十年中，CAP已在多种肿瘤疾病中进行了实验应用，并在体外和体内均显示出抗肿瘤作用。此外，CAP已被证明可提高癌细胞对辐射的敏感性，因此作为当前标准治疗的术中辅助治疗，它可能是提高脊索瘤复发率的有用工具。迄今为止，文献中尚无研究探讨CAP诱导脊索瘤细胞毒性的疗效。我们用CAP处理CH2、CH7和UM-Chor1脊索瘤细胞，测量由此产生的细胞活力和细胞内活性氧积累。在此，我们表明在体外直接暴露于CAP会导致细胞内活性氧和细胞死亡呈剂量依赖性增加，发现脊索瘤细胞对CAP介导的细胞毒性极为敏感。
