Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 38541, Republic of Korea.
Department of Biology, College of Science, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.
Sci Rep. 2017 May 30;7(1):2527. doi: 10.1038/s41598-017-02556-9.
Sound vibration (SV), a mechanical stimulus, can trigger various molecular and physiological changes in plants like gene expression, hormonal modulation, induced antioxidant activity and calcium spiking. It also alters the seed germination and growth of plants. In this study, we investigated the effects of SV on the resistance of Arabidopsis thaliana against Botrytis cinerea infection. The microarray analysis was performed on infected Arabidopsis plants pre-exposed to SV of 1000 Hertz with 100 decibels. Broadly, the transcriptomic analysis revealed up-regulation of several defense and SA-responsive and/or signaling genes. Quantitative real-time PCR (qRT-PCR) analysis of selected genes also validated the induction of SA-mediated response in the infected Arabidopsis plants pre-exposed to SV. Corroboratively, hormonal analysis identified the increased concentration of salicylic acid (SA) in the SV-treated plants after pathogen inoculation. In contrast, jasmonic acid (JA) level in the SV-treated plants remained stable but lower than control plants during the infection. Based on these findings, we propose that SV treatment invigorates the plant defense system by regulating the SA-mediated priming effect, consequently promoting the SV-induced resistance in Arabidopsis against B. cinerea.
声振动（SV）是一种机械刺激，可以在植物中引发各种分子和生理变化，如基因表达、激素调节、诱导抗氧化活性和钙峰。它还会改变种子的萌发和生长。在这项研究中，我们研究了 SV 对拟南芥抵抗 Botrytis cinerea 感染的影响。在将 1000 赫兹、100 分贝的 SV 预先暴露于感染的拟南芥植物后，进行了微阵列分析。总体而言，转录组分析显示，几种防御和 SA 反应性和/或信号基因上调。对选定基因的定量实时 PCR（qRT-PCR）分析也验证了在 SV 预先暴露于感染的拟南芥植物中诱导了 SA 介导的反应。相应地，激素分析确定了在接种病原体后 SV 处理植物中水杨酸（SA）浓度的增加。相比之下，SV 处理植物中的茉莉酸（JA）水平在感染过程中保持稳定，但低于对照植物。基于这些发现，我们提出 SV 处理通过调节 SA 介导的启动效应来增强植物防御系统，从而促进拟南芥对 B. cinerea 的 SV 诱导抗性。
