Prévost Victor, David Karine, Ferrandiz Pedro, Gallet Olivier, Hindié Mathilde
Genodics, SAS, 23 Rue Jean-Jacques Rousseau, 75001 Paris, France.
CY Cergy Paris Université, Biology Department, F-95000, Cergy, France.
Heliyon. 2020 Sep 23;6(9):e04991. doi: 10.1016/j.heliyon.2020.e04991. eCollection 2020 Sep.
Among plant responses to environmentally induced stress modulating protein expression appears to be a key stage in inducible signaling. Our study was focused on an innovative strategy to stimulate plant stress resistance, namely, the use of targeted sequences of specific sound frequencies. The influence of acoustic stimulation on plant protein synthesis was investigated. In our study green peas, , were cultured under hydric stress conditions with targeted acoustic stimulation. Acoustic sequences targeting dehydrins (DHN) which accumulate in plants in response to dehydration were studied. We experimented on pea seeding with two different sequences of sounds: the first one corresponded to DHN cognate protein and the second one was aimed at the DHN consensus sequence. Shoot elongation after pea seed germination was estimated by fresh weight gain studied in the presence of various conditions of exposure to both sequences of sounds. DHN expression in peas was quantified via ELISA tests and Western-blotting by using specific antibodies. A significant increase in fresh weight in peas grown under exposure to the DHN cognate sound sequence was observed, whereas the consensus sound sequence had no effect on growth. Moreover, the 37kDa DHN amount was increased in peas treated with the consensus acoustic sequence. These results suggest that the expression of DHN could be specifically modulated by a designed acoustic stimulus.
在植物对环境诱导胁迫的反应中，调节蛋白质表达似乎是诱导信号传导中的关键阶段。我们的研究集中在一种刺激植物抗逆性的创新策略上，即使用特定声频的靶向序列。研究了声刺激对植物蛋白质合成的影响。在我们的研究中，绿色豌豆在水分胁迫条件下接受靶向声刺激进行培养。研究了靶向脱水素（DHN）的声学序列，脱水素是植物在脱水时积累的蛋白质。我们用两种不同的声音序列对豌豆种子进行了实验：第一种对应于DHN同源蛋白，第二种针对DHN共有序列。通过在两种声音序列的不同暴露条件下研究鲜重增加来估计豌豆种子萌发后的茎伸长。通过ELISA试验和使用特异性抗体的Western印迹法对豌豆中的DHN表达进行定量。观察到在暴露于DHN同源声音序列下生长的豌豆鲜重显著增加，而共有声音序列对生长没有影响。此外，用共有声学序列处理的豌豆中37kDa的DHN量增加。这些结果表明，DHN的表达可以通过设计的声刺激进行特异性调节。
