Chen Xionglin, Zhang Min, Wang Xueer, Chen Yinghua, Yan Yuan, Zhang Lu, Zhang Lin
Key Laboratory of Construction and Detection in Tissue Engineering of Guangdong Province, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical UniversityGuangzhou 510515, China.
Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical UniversityGuangzhou 510515, China.
Am J Transl Res. 2017 May 15;9(5):2352-2362. eCollection 2017.
Cutaneous wound healing following trauma is a complex and dynamic process involving multiple overlapping events following trauma. Two critical elements affecting skin wound healing are neovascularization and inflammation. A nascent vessel can provide nutrition and oxygen to a healing wound. Therefore, treatments strategies that enhance angiogenesis and inhibit inflammation can promote skin wound healing. Previous studies have shown that the SIKVAV peptide (Ser-Ile-Lys-Val-Ala-Val) from laminin can promote angiogenesis . This study evaluated the effects of peptide SIKVAV-modified chitosan hydrogels on skin wound healing. We established skin wounds established in mice and treated them with SIKVAV-modified chitosan hydrogels. H&E staining showed that peptide-modified chitosan hydrogels accelerated the reepithelialization of wounds compared with the negative and positive controls. Immunohistochemistry analysis demonstrated that more myofibroblasts were deposited at wounds treated with peptide-modified chitosan hydrogels that at those treated with negative and positive controls. In addition, peptide-modified chitosan hydrogels promoted angiogenesis as well as keratinocyte proliferation and differentiation, but inhibited inflammation in skin wounds. Taken together, these results suggest that SIKVAV-modified chitosan hydrogels are a promising treatment component for healing-impaired wounds.
创伤后的皮肤伤口愈合是一个复杂且动态的过程，涉及创伤后多个重叠事件。影响皮肤伤口愈合的两个关键因素是新血管形成和炎症。新生血管可为愈合中的伤口提供营养和氧气。因此，促进血管生成和抑制炎症的治疗策略可促进皮肤伤口愈合。先前的研究表明，来自层粘连蛋白的SIKVAV肽（Ser-Ile-Lys-Val-Ala-Val）可促进血管生成。本研究评估了SIKVAV肽修饰的壳聚糖水凝胶对皮肤伤口愈合的影响。我们在小鼠身上制造皮肤伤口，并用SIKVAV肽修饰的壳聚糖水凝胶进行治疗。苏木精-伊红染色显示，与阴性和阳性对照相比，肽修饰的壳聚糖水凝胶加速了伤口的上皮再形成。免疫组织化学分析表明，与阴性和阳性对照处理的伤口相比，肽修饰的壳聚糖水凝胶处理的伤口处沉积了更多的肌成纤维细胞。此外，肽修饰的壳聚糖水凝胶促进血管生成以及角质形成细胞的增殖和分化，但抑制皮肤伤口中的炎症。综上所述，这些结果表明SIKVAV肽修饰的壳聚糖水凝胶是治疗愈合受损伤口的一种有前景的治疗成分。
