Dragostin Oana Maria, Samal Sangram Keshari, Dash Mamoni, Lupascu Florentina, Pânzariu Andreea, Tuchilus Cristina, Ghetu Nicolae, Danciu Mihai, Dubruel Peter, Pieptu Dragos, Vasile Cornelia, Tatia Rodica, Profire Lenuta
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
Laboratory of General Biochemistry and Physical Pharmacy, Centre for Nano- and Biophotonics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
Carbohydr Polym. 2016 May 5;141:28-40. doi: 10.1016/j.carbpol.2015.12.078. Epub 2016 Jan 4.
Chitosan is a non-toxic, biocompatible, biodegradable natural cationic polymer known for its low imunogenicity, antimicrobial, antioxidant effects and wound-healing activity. To improve its therapeutic potential, new chitosan-sulfonamide derivatives have been designed to develop new wound dressing biomaterials. The structural, morphological and physico-chemical properties of synthesized chitosan derivatives were analyzed by FT-IR, (1)H NMR spectroscopy, scanning electron microscopy, swelling ability and porosity. Antimicrobial, in vivo testing and biodegradation behavior have been also performed. The chitosan derivative membranes showed improved swelling and biodegradation rate, which are important characteristics required for the wound healing process. The antimicrobial assay evidenced that chitosan-based sulfadiazine, sulfadimethoxine and sulfamethoxazole derivatives were the most active. The MTT assay showed that some of chitosan derivatives are nontoxic. Furthermore, the in vivo study on burn wound model induced in Wistar rats demonstrated an improved healing effect and enhanced epithelialization of chitosan-sulfonamide derivatives compared to neat chitosan. The obtained results strongly recommend the use of some of the newly developed chitosan derivatives as antimicrobial wound dressing biomaterials.
壳聚糖是一种无毒、生物相容、可生物降解的天然阳离子聚合物，以其低免疫原性、抗菌、抗氧化作用及伤口愈合活性而闻名。为提高其治疗潜力，已设计出新型壳聚糖 - 磺胺衍生物以开发新型伤口敷料生物材料。通过傅里叶变换红外光谱（FT - IR）、核磁共振氢谱（¹H NMR）、扫描电子显微镜、溶胀能力和孔隙率分析了合成的壳聚糖衍生物的结构、形态和物理化学性质。还进行了抗菌、体内测试和生物降解行为研究。壳聚糖衍生物膜显示出改善的溶胀和生物降解速率，这是伤口愈合过程所需的重要特性。抗菌试验证明，基于壳聚糖的磺胺嘧啶、磺胺二甲氧嘧啶和磺胺甲恶唑衍生物活性最强。MTT试验表明一些壳聚糖衍生物无毒。此外，对Wistar大鼠诱导的烧伤伤口模型进行的体内研究表明，与纯壳聚糖相比，壳聚糖 - 磺胺衍生物具有更好的愈合效果和增强的上皮形成。所得结果强烈推荐使用一些新开发的壳聚糖衍生物作为抗菌伤口敷料生物材料。
