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山竹果实成熟的RNA测序分析

RNA-seq analysis of mangosteen ( L.) fruit ripening.

作者信息

Abdul-Rahman Azhani, Goh Hoe-Han, Loke Kok-Keong, Noor Normah Mohd, Aizat Wan Mohd

机构信息

Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

出版信息

Genom Data. 2017 May 13;12:159-160. doi: 10.1016/j.gdata.2017.05.013. eCollection 2017 Jun.

Abstract

Mangosteen ( L.) is known for its delectable taste and contains high amount of xanthones which have been reported to possess anti-cancer, anti-inflammatory and other bioactive properties. However, stage-specific regulation of mangosteen fruit ripening has never been studied in detail. We have performed a comparative transcriptomic analysis of three ripening stages (Stage 0, 2 and 6) of mangosteen. We have obtained a raw data from six libraries through Illumina HiSeq 4000. A total of ~ 40 Gb of raw data were generated. Clean reads of 650,887,650 (bp) were obtained from 656,913,570 (bp) raw reads. The raw transcriptome data were deposited to SRA database, with the BioProject accession number of PRJNA339916. These data will be beneficial for transcriptome profiling in order to study the regulation of mangosteen fruit ripening. The lack of a complete sequence database from this species impedes protein identification. These data sets provide a reference data for the exploration of novel genes or proteins to understand mangosteen fruit ripening behaviour.

摘要

山竹(莽吉柿)以其美味的口感而闻名,并且含有大量的氧杂蒽酮,据报道这些氧杂蒽酮具有抗癌、抗炎和其他生物活性特性。然而,山竹果实成熟的阶段特异性调控从未得到详细研究。我们对山竹的三个成熟阶段(阶段0、2和6)进行了比较转录组分析。我们通过Illumina HiSeq 4000从六个文库中获得了原始数据。共产生了约40Gb的原始数据。从656,913,570(bp)的原始读段中获得了650,887,650(bp)的干净读段。原始转录组数据已存入SRA数据库,生物项目登录号为PRJNA339916。这些数据将有助于进行转录组分析,以研究山竹果实成熟的调控。该物种缺乏完整的序列数据库阻碍了蛋白质鉴定。这些数据集为探索新基因或蛋白质以了解山竹果实成熟行为提供了参考数据。

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