Wang Xiaoxu, Sun Lina, Wang Hui, Wu Hao, Chen Su, Zheng Xuehao
a Key Laboratory of Regional Environment and Eco-Remediation, Ministry of Education , Shenyang University , Shenyang , Liaoning Province , People's Republic of China.
Environ Technol. 2018 Jul;39(13):1733-1744. doi: 10.1080/09593330.2017.1337235. Epub 2017 Jun 9.
Field-scale bioremediation of dichlorodiphenyl trichloroethanes (DDTs) and polycyclic aromatic hydrocarbons (PAHs) contaminated farmland soil from the Shenyang North New Area of China was studied using the bacteria Arthrobacter globiformis. The additive effects of different concentrations of biosurfactant rhamnolipids (RLs) and anionic-nonionic mixed surfactant (SDBS-Tween 80) were evaluated. DDT and PAH removal rates by A. globiformis after 150 days of remediation were 52.1% and 21.9%, respectively. At the optimum RL concentration of 5 mg kg, DDTs and PAHs had removal rates of 64.3% and 35.6%, respectively, at 150 days. This was 60.7% and 29.3% higher than the control; 36.9% and 19.8% higher than soil with RL-5 alone; and 12.2% and 13.8% higher than the A. globiformis treatment alone. RL-5 can enhance soil enzyme activity and A. globiformis reproduction during the DDT and PAH biodegradation processes. This study illustrates a highly efficient, low-cost in situ soil bioremediation technology that could have practical utility.
利用球形节杆菌，对中国沈阳北部新区受二氯二苯三氯乙烷（DDTs）和多环芳烃（PAHs）污染的农田土壤进行田间规模的生物修复研究。评估了不同浓度的生物表面活性剂鼠李糖脂（RLs）和阴离子 - 非离子混合表面活性剂（SDBS - Tween 80）的增效作用。修复150天后，球形节杆菌对DDT和PAH的去除率分别为52.1%和21.9%。在鼠李糖脂最佳浓度为5 mg/kg时，150天时DDTs和PAHs的去除率分别为64.3%和35.6%。这比对照组分别高出60.7%和29.3%；比单独添加RL - 5的土壤分别高出36.9%和19.8%；比单独使用球形节杆菌处理分别高出12.2%和13.8%。在DDT和PAH生物降解过程中，RL - 5可以提高土壤酶活性和球形节杆菌的繁殖能力。本研究阐明了一种高效、低成本的原位土壤生物修复技术，该技术具有实际应用价值。
