Department of Botany and Microbiology, Gurukula Kangri (Deemed To Be University), Haridwar, 249404, Uttarakhand, India.
Arch Microbiol. 2021 Jul;203(5):2689-2698. doi: 10.1007/s00203-021-02250-5. Epub 2021 Mar 14.
A phytohormone producing, N-fixing and 1-aminocyclopropane-1-carboxylate (ACC) deaminase synthesizing bacterium Ensifer adhaerens KS23 effectively increased the yield and nutritional contents of Pisum sativum var. Arkel. The isolate KS23 showed positive ACC deaminase activity with 174.2 (nmol of α-ketobutyrate/g-1 biomass) a 9.7-fold increase in glutathione S-transferase activity. The proximate analysis exhibited an increased yield of protein (21.45%), carbohydrate (38.90%), sulphur (29.94%) starch (27.52%), total ash (35.57%), fat content (27.5%), nitrogen (24.06%) and hydrogen (17.91%) in treated seeds of P. sativum as compared to untreated crop seeds in field trials at Srikot village, Srinagar-246,174 (Garhwal) India. The most desirable essential and non-essential amino-acids content was also enhanced simultaneously by E. adhaerens KS23 as compared to non-treated crop seeds. This study revealed the enhancement of various nutritional contents resulting in quality improvement and an increase in growth productivity of pea. This study provides an encouraging result that may benefit the marginal income of farmers belonging mainly to hilly regions who are dependent on traditional methods of farming and thus improving their economy.
产植物激素、固氮和 1-氨基环丙烷-1-羧酸（ACC）脱氨酶的根瘤菌 Ensifer adhaerens KS23 可有效提高豌豆（Pisum sativum var. Arkel）的产量和营养价值。该分离株 KS23 表现出阳性 ACC 脱氨酶活性，谷胱甘肽 S-转移酶活性增加了 9.7 倍，达到 174.2（nmol α-酮丁酸/g-1 生物质）。在印度斯利科特村（Srikot village）的田间试验中，与未处理的作物种子相比，处理过的豌豆种子的粗蛋白（21.45%）、碳水化合物（38.90%）、硫（29.94%）、淀粉（27.52%）、总灰分（35.57%）、脂肪含量（27.5%）、氮（24.06%）和氢（17.91%）的产量均有所增加。同时，与未处理的作物种子相比，根瘤菌 KS23 还同时提高了各种必需和非必需氨基酸的含量。本研究提高了豌豆的各种营养成分，从而改善了豌豆的品质并提高了其生长生产力。本研究提供了一个令人鼓舞的结果，可能有利于主要依赖传统农业方法的山地农民增加收入，从而改善他们的经济状况。
