Vogler Alfried P, DeSalle Rob
American Museum of Natural History, Central Park West at 79th Street, New York, New York, 10024.
Evolution. 1993 Aug;47(4):1192-1202. doi: 10.1111/j.1558-5646.1993.tb02146.x.
Genetic variation was assessed in the tiger beetle, Cicindela dorsalis, by sequencing of three regions of the mtDNA genome. Populations of four morphologically distinguishable subspecies were sampled from 28 representative locations covering almost the entire geographic range of the species in coastal North America. In 78 individuals analyzed for 656 base pairs from four different genes, 17 different haplotypes could be distinguished. A cladistic analysis grouped the haplotype sequences into two main lineages, one from the Atlantic Ocean and one from the Gulf of Mexico. Haplotypes within the two clades were very similar to each other. Most of the characters that distinguished these closely related haplotypes were homoplastic. The geographic distribution of haplotypes did not coincide with the distribution of morphological subspecies, but no evidence for hybridization between two subspecies could be inferred from this observation. The implications of these findings for the evolution of gene sequences at and below the species level are discussed.
通过对线粒体DNA基因组三个区域进行测序，评估了虎甲（Cicindela dorsalis）的遗传变异。从28个具有代表性的地点采集了四个形态上可区分的亚种的种群，这些地点几乎覆盖了该物种在北美沿海的整个地理范围。在对78个个体的四个不同基因的656个碱基对进行分析时，可以区分出17种不同的单倍型。系统发育分析将单倍型序列分为两个主要谱系，一个来自大西洋，一个来自墨西哥湾。两个分支内的单倍型彼此非常相似。区分这些密切相关单倍型的大多数特征都是同塑性的。单倍型的地理分布与形态亚种的分布不一致，但从这一观察结果中无法推断出两个亚种之间杂交的证据。讨论了这些发现对物种水平及以下基因序列进化的影响。
