Lee D H, Lee J D
Korean J Intern Med. 1988 Jan;3(1):24-9. doi: 10.3904/kjim.1988.3.1.24.
The distribution of hepatitis B virus (HBV) DNA in tumor tissue sections from six Korean patients with HBsAg positive hepatocellular carcinoma (HCC) was examined by in situ hybridization using a biotin-labeled recombinant, cloned HBV DNA probe. All patients tested were positive for both HBeAg and anti-HBc in their sera. HBV DNA was distributed abundantly in the cytoplasm and rarely in the nuclei of tumor cells. The validity of the in situ hybridization assay was confirmed by the dot blotting technique using a P-labeled HBV DNA probe obtained by nick translation. In conclusion, it is speculated that integration of HBV DNA into host DNA as well as persistant amplified replication of the HBV DNA within the hepatocytes is linked etiologically to the development of human hepatocellular carcinoma.
采用生物素标记的重组克隆乙肝病毒（HBV）DNA探针，通过原位杂交技术检测了6例乙肝表面抗原（HBsAg）阳性的韩国肝细胞癌（HCC）患者肿瘤组织切片中HBV DNA的分布情况。所有检测患者血清中的乙肝e抗原（HBeAg）和乙肝核心抗体（抗-HBc）均呈阳性。HBV DNA大量分布于肿瘤细胞的细胞质中，细胞核中则很少见。通过缺口平移法获得的P标记HBV DNA探针斑点杂交技术，证实了原位杂交检测方法的有效性。总之，推测HBV DNA整合入宿主DNA以及在肝细胞内持续扩增复制在病因学上与人类肝细胞癌的发生有关。
