Kusaka Y, Muraoka A, Tokiwa T, Sato J
Division of Pathology, Cancer Institute, Medical School, Okayama University.
Hum Cell. 1988 Mar;1(1):92-4.
A cell line, HuH-28 was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has been in continuous culture over 10 month period with slow growth potential. HuH-28 was composed of spindle-shaped cells as major population besides a small percentage of polygonal-shaped cells. Chromosome number of the cells were distributed near the hypotriploid region on the 3rd passage. HuH-28 cells were not transplantable into nude mice, but secreted some tumor markers including alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), beta 2-microglobulin (BMG), ferritin, elastase-1 and tissue polypeptide antigen (TPA). This HuH-28 cell line will represent a good model for the investigation of carcinogenesis, histogenesis+ and diagnosis of CCC.
一种细胞系HuH-28是从一名胆管细胞癌（CCC）患者体内体外建立的。该细胞系已经连续培养了10个月，生长潜力缓慢。HuH-28主要由纺锤形细胞组成，此外还有一小部分多边形细胞。在第3代时，细胞的染色体数分布在亚三倍体区域附近。HuH-28细胞不能移植到裸鼠体内，但能分泌一些肿瘤标志物，包括碱性磷酸酶（ALP）、γ-谷氨酰转肽酶（GGT）、β2-微球蛋白（BMG）、铁蛋白、弹性蛋白酶-1和组织多肽抗原（TPA）。这种HuH-28细胞系将为研究CCC的致癌机制、组织发生和诊断提供一个良好的模型。
