Su Guangyue, Chen Gang, An Xiao, Wang Haifeng, Pei Yue-Hu
Department of Traditional Chinese Materia Medica, Shenyang Pharmaceutical UniversityShenyang, China.
Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical UniversityShenyang, China.
Front Pharmacol. 2017 May 17;8:271. doi: 10.3389/fphar.2017.00271. eCollection 2017.
Baicalin is the main bioactive flavonoid constituent isolated from . The mechanisms of protection of liver remain unclear. In this study, H NMR-based metabonomics approach has been used to investigate the alleviation effect of Baicalin. H NMR metabolomics analyses of urine and serum from rats, was performed to illuminate the alleviation effect of Baicalin on mineral medicine (cinnabar)-induced liver and kidney toxicity. The metabolic profiles of groups receiving Baicalin at a dose of 80 mg/kg were remarkably different from cinnabar, and meanwhile, the level of endogenous metabolites returned to normal compared to group cinnabar. PLS-DA scores plots demonstrated that the variation tendency of control and Baicalein are apart from Cinnabar. The metabolic profiles of group Baicalein were similar to those of group control. Statistics results were confirmed by the histopathological examination and biochemical assay. Baicalin have the alleviation effect to the liver and kidney damage induced by cinnabar. The Baicalin could regulate endogenous metabolites associated with the energy metabolism, choline metabolism, amino acid metabolism, and gut flora.
黄芩苷是从……中分离出的主要生物活性黄酮类成分。其肝脏保护机制尚不清楚。在本研究中，基于氢核磁共振的代谢组学方法被用于研究黄芩苷的缓解作用。对大鼠尿液和血清进行氢核磁共振代谢组学分析，以阐明黄芩苷对矿物药（朱砂）诱导的肝肾毒性的缓解作用。接受80mg/kg剂量黄芩苷组的代谢谱与朱砂组显著不同，同时，与朱砂组相比，内源性代谢物水平恢复正常。偏最小二乘判别分析得分图表明，对照组和黄芩苷组的变化趋势与朱砂组不同。黄芩苷组的代谢谱与对照组相似。统计结果通过组织病理学检查和生化分析得到证实。黄芩苷对朱砂诱导的肝肾损伤具有缓解作用。黄芩苷可调节与能量代谢、胆碱代谢、氨基酸代谢和肠道菌群相关的内源性代谢物。
