Colosia A D, Lively M, el-Maghrabi M R, Pilkis S J
Biochem Biophys Res Commun. 1987 Mar 30;143(3):1092-8. doi: 10.1016/0006-291x(87)90364-0.
A cDNA clone for rat liver 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was isolated from a lambda gt11 rat liver expression library by antibody screening. The clone was approximately 1100 bases in length and the derived amino acid sequence contained 303 residues at the carboxyl end of the subunit. This derived amino acid sequence corresponded exactly with the actual amino acid sequence of the enzyme determined by direct sequencing of the protein.
通过抗体筛选，从λgt11大鼠肝脏表达文库中分离出大鼠肝脏6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶的cDNA克隆。该克隆长度约为1100个碱基，推导的氨基酸序列在亚基的羧基末端包含303个残基。此推导的氨基酸序列与通过蛋白质直接测序确定的该酶的实际氨基酸序列完全一致。
