Cai Keying, Zeng Mulan, Liu Fenfen, Liu Nan, Huang Zhenzhong, Song Yonghai, Wang Li
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, China.
Luminescence. 2017 Nov;32(7):1277-1282. doi: 10.1002/bio.3321. Epub 2017 Jun 1.
An easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent BSA-AuNPs@Tb-AMP (BSA, bovine serum albumin; AMP, adenosine 5'-monophosphate; AuNPs, Au nanoparticles) metal-organic framework (MOF) nanostructures were synthesized by encapsulating BSA-AuNPs into Tb-AMP MOFs for the detection of 2,6-pyridinedicarboxylic acid (DPA) and Hg . DPA could strongly co-ordinate with Tb to replace water molecules from the Tb center and accordingly enhanced the fluorescence of Tb-AMP MOFs. The fluorescence of BSA-AuNPs at 405 nm remained constant. While the fluorescence of BSA-AuNPs at 635 nm was quenched after Hg was added, the fluorescence of Tb-AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg . The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F /F linearly increased with increase of DPA concentration in the range of 50 nM to 10 μM with a detection limit as low as 17.4 nM. F /F increased linearly with increase of Hg concentration ranging from 50 nM to 1 μM with a detection limit as low as 20.9 nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg in running water.
本工作展示了一种合成比率荧光纳米传感器的简便有效策略。通过将牛血清白蛋白修饰的金纳米粒子（BSA-AuNPs）包裹到铽-5'-单磷酸腺苷金属有机框架（Tb-AMP MOF）中，合成了新型荧光纳米结构BSA-AuNPs@Tb-AMP（BSA，牛血清白蛋白；AMP，5'-单磷酸腺苷；AuNPs，金纳米粒子），用于检测2,6-吡啶二甲酸（DPA）和汞离子（Hg²⁺）。DPA能与铽强烈配位，从铽中心取代水分子，从而增强Tb-AMP MOF的荧光。405nm处BSA-AuNPs的荧光保持恒定。加入Hg²⁺后，635nm处BSA-AuNPs的荧光猝灭，而Tb-AMP MOF的荧光保持不变。据此，构建了一种用于检测DPA和Hg²⁺的比率荧光纳米传感器。该比率纳米传感器对DPA具有良好的选择性，优于其他物质。在50 nM至10 μM范围内，F₆₃₅/F₄₀₅随DPA浓度增加呈线性增加，检测限低至17.4 nM。在50 nM至1 μM范围内，F₆₃₅/F₄₀₅随Hg²⁺浓度增加呈线性增加，检测限低至20.9 nM。此外，该纳米传感器可成功应用于自来水中DPA和Hg²⁺的测定。
