Fujita Moeka, Kusajima Miyuki, Okumura Yasuko, Nakajima Masami, Minamisawa Kiwamu, Nakashita Hideo
a Research Center for Bioresources Development, Faculty of Biotechnology , Fukui Prefectural University , Awara , Japan.
b Faculty of Agriculture , Ibaraki University , Ami , Japan.
Biosci Biotechnol Biochem. 2017 Aug;81(8):1657-1662. doi: 10.1080/09168451.2017.1329621. Epub 2017 Jun 1.
A plant growth-promoting bacteria, Azospirillum sp. B510, isolated from rice, can enhance growth and yield and induce disease resistance against various types of diseases in rice. Because little is known about the interaction between other plant species and this strain, we have investigated the effect of its colonization on disease resistance in tomato plants. Treatment with this strain by soil-drenching method established endophytic colonization in root tissues in tomato plant. The endophytic colonization with this strain-induced disease resistance in tomato plant against bacterial leaf spot caused by Pseudomonas syringae pv. tomato and gray mold caused by Botrytis cinerea. In Azospirillum-treated plants, neither the accumulation of SA nor the expression of defense-related genes was observed. These indicate that endophytic colonization with Azospirillum sp. B510 is able to activate the innate immune system also in tomato, which does not seem to be systemic acquired resistance.
从水稻中分离出的一种促进植物生长的细菌——固氮螺菌属（Azospirillum）B510菌株，能够促进水稻生长、提高产量，并诱导水稻对多种病害产生抗病性。由于对该菌株与其他植物物种之间的相互作用了解甚少，我们研究了其定殖对番茄植株抗病性的影响。通过土壤浇灌法用该菌株处理后，在番茄植株的根组织中建立了内生定殖。该菌株的内生定殖诱导番茄植株对丁香假单胞菌番茄致病变种（Pseudomonas syringae pv. tomato）引起的细菌性叶斑病和灰葡萄孢（Botrytis cinerea）引起的灰霉病产生抗病性。在用固氮螺菌处理的植株中，未观察到水杨酸（SA）的积累和防御相关基因的表达。这些结果表明，固氮螺菌属B510菌株的内生定殖也能够激活番茄的先天免疫系统，这似乎不是系统获得性抗性。
