Gong Hai, Agbana Temitope E, Pozzi Paolo, Soloviev Oleg, Verhaegen Michel, Vdovin Gleb
Opt Lett. 2017 Jun 1;42(11):2122-2125. doi: 10.1364/OL.42.002122.
In this Letter, we show that a Shack-Hartmann wavefront sensor can be used for the quantitative measurement of the specimen optical path difference (OPD) in an ordinary incoherent optical microscope, if the spatial coherence of the illumination light in the plane of the specimen is larger than the microscope resolution. To satisfy this condition, the illumination numerical aperture should be smaller than the numerical aperture of the imaging lens. This principle has been successfully applied to build a high-resolution reference-free instrument for the characterization of the OPD of micro-optical components and microscopic biological samples.
在本信函中，我们表明，如果样品平面上照明光的空间相干性大于显微镜分辨率，那么夏克-哈特曼波前传感器可用于普通非相干光学显微镜中样品光程差（OPD）的定量测量。为满足此条件，照明数值孔径应小于成像透镜的数值孔径。这一原理已成功应用于构建一种用于表征微光学元件和微观生物样品OPD的高分辨率无参考仪器。
