Mason Shayne, Reinecke Carolus J, Solomons Regan, Wevers Ron A, Engelke Udo F H
Centre for Human Metabolomics, Faculty of Natural Sciences, North-West University (Potchefstroom Campus), Private Bag X6001, Potchefstroom, South Africa.
Centre for Human Metabolomics, Faculty of Natural Sciences, North-West University (Potchefstroom Campus), Private Bag X6001, Potchefstroom, South Africa.
J Pharm Biomed Anal. 2017 Sep 5;143:56-61. doi: 10.1016/j.jpba.2017.04.054. Epub 2017 May 25.
Exploratory metabolomics studies of cerebrospinal fluid (CSF), using proton nuclear magnetic resonance (H NMR) spectroscopy, hold major potential application in neurodiagnostics. Such studies, however, rely upon established databases of known metabolites. Here we address the 'unknowns' in the H NMR spectra of CSF from treated pediatric meningitis cases. Through knowledge of the clinical information given by the pediatrician and analytical application of H NMR spectroscopy on pure reference compounds of the medication used, we identified four of the previously unknown compounds in the H NMR CSF spectra - the drugs pyrazinamide, isoniazid, acyclovir, and sulfamethoxazole. We report on the one- and two-dimensional H NMR spectral data and chemical information of these four compounds. By expanding our knowledge of H NMR CSF spectra from treated meningitis cases, we are able to bring H NMR closer to the forefront of neurodiagnostics.
利用质子核磁共振（H NMR）光谱对脑脊液（CSF）进行的探索性代谢组学研究在神经诊断中具有重大潜在应用价值。然而，此类研究依赖于已知代谢物的既定数据库。在此，我们探讨了经治疗的小儿脑膜炎病例脑脊液H NMR光谱中的“未知物”。通过了解儿科医生提供的临床信息，并将H NMR光谱分析应用于所用药物的纯参考化合物，我们在脑脊液H NMR光谱中鉴定出了四种先前未知的化合物——药物吡嗪酰胺、异烟肼、阿昔洛韦和磺胺甲恶唑。我们报告了这四种化合物的一维和二维H NMR光谱数据及化学信息。通过扩展我们对经治疗的脑膜炎病例脑脊液H NMR光谱的认识，我们能够使H NMR更接近神经诊断的前沿。
