粗糙脉孢菌中谷氨酰胺降解的ω-酰胺酶途径。
Omega-amidase pathway in the degradation of glutamine in Neurospora crassa.
作者信息
Calderón J, Morett E, Mora J
出版信息
J Bacteriol. 1985 Feb;161(2):807-9. doi: 10.1128/jb.161.2.807-809.1985.
Abstract
Evidence for the participation of the glutamine transaminase-omega-amidase pathway in the utilization of glutamine in Neurospora crassa was obtained. Its participation is indicated by the in vitro activities of glutamine transaminase and omega-amidase, the in vivo accumulation of alpha-ketoglutaramate when an inhibitor of transamidases is present, and the inhibition by aminooxyacetic acid and 6-diazo-5-oxo-L-norleucine of the ammonium excreted in the presence of glutamine by a mutant strain that lacks glutamate dehydrogenase and glutamate synthase.
摘要
获得了谷氨酰胺转氨酶-ω-酰胺酶途径参与粗糙脉孢菌中谷氨酰胺利用的证据。谷氨酰胺转氨酶和ω-酰胺酶的体外活性、转酰胺酶抑制剂存在时α-酮戊二酸在体内的积累，以及氨基氧乙酸和6-重氮-5-氧代-L-正亮氨酸对缺乏谷氨酸脱氢酶和谷氨酸合酶的突变菌株在谷氨酰胺存在时排泄铵的抑制作用，均表明了该途径的参与。
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