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促进恶性黑色素瘤干细胞状态的特定底物的组合发现。

Combinatorial Discovery of Defined Substrates That Promote a Stem Cell State in Malignant Melanoma.

作者信息

Zhang Douglas, Lee Junmin, Sun Michael B, Pei Yi, Chu James, Gillette Martha U, Fan Timothy M, Kilian Kristopher A

机构信息

Department of Materials Science and Engineering, Department of Cell and Developmental Biology, Department of Veterinary Clinical Medicine, and Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

出版信息

ACS Cent Sci. 2017 May 24;3(5):381-393. doi: 10.1021/acscentsci.6b00329. Epub 2017 Apr 26.

DOI:10.1021/acscentsci.6b00329
PMID:28573199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5445527/
Abstract

The tumor microenvironment is implicated in orchestrating cancer cell transformation and metastasis. However, specific cell-ligand interactions between cancer cells and the extracellular matrix are difficult to decipher due to a dynamic and multivariate presentation of many signaling molecules. Here we report a versatile peptide microarray platform that is capable of screening for cancer cell phenotypic changes in response to ligand-receptor interactions. Using a screen of 78 peptide combinations derived from proteins present in the melanoma microenvironment, we identify a proteoglycan binding and bone morphogenic protein 7 (BMP7) derived sequence that selectively promotes the expression of several putative melanoma initiating cell markers. We characterize signaling associated with each of these peptides in the activation of melanoma pro-tumorigenic signaling and reveal a role for proteoglycan mediated adhesion and signaling through Smad 2/3. A defined substratum that controls the state of malignant melanoma may prove useful in spatially normalizing a heterogeneous population of tumor cells for discovery of therapeutics that target a specific state and for identifying new drug targets and reagents for intervention.

摘要

肿瘤微环境与癌细胞的转化和转移密切相关。然而，由于许多信号分子呈现出动态和多变量的特点，癌细胞与细胞外基质之间特定的细胞-配体相互作用难以解析。在此，我们报告了一种多功能肽微阵列平台，该平台能够筛选癌细胞因配体-受体相互作用而产生的表型变化。通过对源自黑色素瘤微环境中存在的蛋白质的78种肽组合进行筛选，我们鉴定出一种蛋白聚糖结合序列和源自骨形态发生蛋白7（BMP7）的序列，该序列可选择性地促进几种假定的黑色素瘤起始细胞标志物的表达。我们对这些肽在激活黑色素瘤促肿瘤信号传导过程中相关的信号进行了表征，并揭示了蛋白聚糖介导的黏附作用以及通过Smad 2/3进行信号传导的作用。一种能够控制恶性黑色素瘤状态的特定基质，可能有助于在空间上使异质性肿瘤细胞群体正常化，从而发现针对特定状态的治疗方法，并识别用于干预的新药物靶点和试剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/4aaa8def490f/oc-2016-00329s_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/910641c8c5d8/oc-2016-00329s_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/d76743744452/oc-2016-00329s_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/b782ee871e64/oc-2016-00329s_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/abd5c3f26fa3/oc-2016-00329s_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/2f76721f8a11/oc-2016-00329s_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/4aaa8def490f/oc-2016-00329s_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/910641c8c5d8/oc-2016-00329s_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/d76743744452/oc-2016-00329s_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/b782ee871e64/oc-2016-00329s_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/abd5c3f26fa3/oc-2016-00329s_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/2f76721f8a11/oc-2016-00329s_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c059/5445527/4aaa8def490f/oc-2016-00329s_0006.jpg

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本文引用的文献

1
Peptide microarrays for the discovery of bioactive surfaces that guide cellular processes: a single step azide-alkyne "click" chemistry approach.用于发现引导细胞过程的生物活性表面的肽微阵列：一种单步叠氮化物-炔烃“点击”化学方法。
J Mater Chem B. 2014 Jul 21;2(27):4280-4288. doi: 10.1039/c4tb00375f. Epub 2014 Jun 4.
2
Melanoma.黑色素瘤。
Nat Rev Dis Primers. 2015 Apr 23;1:15003. doi: 10.1038/nrdp.2015.3.
3
Interfacial geometry dictates cancer cell tumorigenicity.界面几何决定癌细胞的肿瘤形成能力。
可调节的合成细胞外基质用于研究乳腺癌对生物物理和生化信号的反应。
APL Bioeng. 2019 Feb 8;3(1):016101. doi: 10.1063/1.5064596. eCollection 2019 Mar.
Nat Mater. 2016 Aug;15(8):856-62. doi: 10.1038/nmat4610. Epub 2016 Apr 4.
4
RGD and BMP-2 mimetic peptide crosstalk enhances osteogenic commitment of human bone marrow stem cells.RGD与骨形态发生蛋白-2模拟肽的相互作用增强人骨髓干细胞的成骨定向分化。
Acta Biomater. 2016 May;36:132-42. doi: 10.1016/j.actbio.2016.03.032. Epub 2016 Mar 18.
5
Cell shape and the presentation of adhesion ligands guide smooth muscle myogenesis.细胞形状和黏附配体的呈现引导平滑肌肌生成。
J Biomed Mater Res A. 2016 May;104(5):1212-20. doi: 10.1002/jbm.a.35661. Epub 2016 Feb 11.
6
Glycosylation in cancer: mechanisms and clinical implications.癌症中的糖基化：机制与临床意义。
Nat Rev Cancer. 2015 Sep;15(9):540-55. doi: 10.1038/nrc3982. Epub 2015 Aug 20.
7
TGF-β/BMP signaling and other molecular events: regulation of osteoblastogenesis and bone formation.TGF-β/BMP 信号转导及其他分子事件：成骨细胞生成和骨形成的调控。
Bone Res. 2015 Apr 14;3:15005. doi: 10.1038/boneres.2015.5. eCollection 2015.
8
A cell-ECM screening method to predict breast cancer metastasis.一种预测乳腺癌转移的细胞-细胞外基质筛选方法。
Integr Biol (Camb). 2015 Feb;7(2):198-212. doi: 10.1039/c4ib00218k.
9
Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development.新型骨形态发生蛋白信号通过 Smad2 和 Smad3 调节癌症的进展和发展。
FASEB J. 2014 Mar;28(3):1248-67. doi: 10.1096/fj.13-239178. Epub 2013 Dec 5.
10
The role played by the microenvironment in site-specific metastasis.微环境在特定部位转移中所起的作用。
Cancer Lett. 2014 Sep 28;352(1):54-8. doi: 10.1016/j.canlet.2013.08.029. Epub 2013 Aug 27.