艾灸通过促进细胞凋亡和抑制增殖相关癌基因减轻大鼠胃癌前病变。

MOXIBUSTION ALLEVIATES GASTRIC PRECANCEROUS LESIONS IN RATS BY PROMOTING CELL APOPTOSIS AND INHIBITING PROLIFERATION-RELATED ONCOGENES.

作者信息

Peng Li, Xie Yu-Feng, Wang Chen-Guang, Wu Huan-Gan, Liu Mi, Wang Ya-Dong, Ma Fu-Qiang, Chang Xiao-Rong, Yang Zong-Bao

机构信息

Medical College, Xiamen University, Xiamen 361102 PRC.

Acupuncture and Moxibustion Department, Shenzhen 2 hospital of Traditional Chinese Medicine (Futian hospital of Traditional Chinese Medicine), Shenzhen 518000 PRC.

出版信息

Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):148-160. doi: 10.21010/ajtcam.v14i2.16. eCollection 2017.

DOI:10.21010/ajtcam.v14i2.16

PMID:28573231

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5446438/

Abstract

BACKGROUND

It is well known that gastric mucosa dysplasia and intestinal metaplasia are gastric precancerous lesions (GPL). Moxibustion treatment of (ST21) and (ST36) alleviated the inflammatory response and dysplasia of gastric mucosa in our previous study. The purpose of this study was to further examine the underlying mechanism of moxibustion treatment of ST21 and ST36 on GPL.

MATERIALS AND METHODS

Sixty SD rats were divided into five groups and rats with GPL were treated with either moxibustion (ST), moxibustion (Sham), or vitacoenzyme. B-cell lymphoma 2 (bcl-2), tumor protein p53 (P53) and cellular Myc (C-MYC), which are related to cell apoptosis, proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), argyrophilic nucleolar organizer region proteins (Ag-NORs), which are associated with cell proliferation, and cell signaling proteins, nuclear factor kappa B (NF-κB), epidermal growth factor receptor (EGFR) and phosphorylated extracellular signal regulated kinase (p-ERK), were measured after moxibustion treatment.

RESULTS

Compared with Control group, gastric mucosa in GPL group showed abnormal mucosal proliferation and pathological mitotic figure, the mRNA expression of bcl-2, P53 and C-MYC increased significantly ( < 0.01), the protein expression of PCNA, VEGF, Ag-NORs and the activity of NF-κβ as well as EGFR/ERK signaling proteins also increased significantly ( < 0.01). Moxibustion treatment decreased gastric mucosal proliferation and pathological mitotic figure, down-regulated the mRNA expression of bcl-2, P53, C-MYC ( < 0.01), decreased the protein expression of PCNA, VEGF, Ag-NORs and the activity of NF-κβ as well as EGFR/ERK signaling proteins significantly ( < 0.01). But moxibustion treatment of Sham didn't show the same effect on GPL.

CONCLUSION

Moxibustion treatment inhibited cell apoptosis and reduced gastric mucosa dysplasia by inhibiting the expression of bcl-2, P53, C-MYC and decreased the activity of NF-κβ as well as EGFR/ERK signaling proteins.

摘要

背景

众所周知，胃黏膜发育异常和肠化生是胃癌前病变（GPL）。在我们之前的研究中，艾灸足三里（ST36）和中脘（ST21）可减轻胃黏膜的炎症反应和发育异常。本研究旨在进一步探讨艾灸ST21和ST36治疗GPL的潜在机制。

材料与方法

将60只SD大鼠分为五组，对患有GPL的大鼠分别进行艾灸（ST）、假艾灸（Sham）或给予维生素辅酶治疗。艾灸治疗后检测与细胞凋亡相关的B细胞淋巴瘤-2（bcl-2）、肿瘤蛋白p53（P53）和细胞Myc（C-MYC），与细胞增殖相关的增殖细胞核抗原（PCNA）、血管内皮生长因子（VEGF）、嗜银核仁组织区蛋白（Ag-NORs），以及细胞信号蛋白核因子κB（NF-κB）、表皮生长因子受体（EGFR）和磷酸化细胞外信号调节激酶（p-ERK）。

结果

与对照组相比，GPL组胃黏膜出现异常黏膜增殖和病理性有丝分裂象，bcl-2、P53和C-MYC的mRNA表达显著增加（P<0.01），PCNA、VEGF、Ag-NORs的蛋白表达以及NF-κβ的活性以及EGFR/ERK信号蛋白也显著增加（P<0.01）。艾灸治疗可减少胃黏膜增殖和病理性有丝分裂象，下调bcl-2、P53、C-MYC的mRNA表达（P<0.01），显著降低PCNA、VEGF、Ag-NORs的蛋白表达以及NF-κβ的活性以及EGFR/ERK信号蛋白（P<0.01）。但假艾灸治疗对GPL没有显示出相同的效果。