Diederichs Kay, Wang Meitian
Department of Biology, Universität Konstanz, Box 647, D-78457, Konstanz, Germany.
Swiss Light Source, Paul Scherrer Institute, CH-5232, Villigen, Switzerland.
Methods Mol Biol. 2017;1607:239-272. doi: 10.1007/978-1-4939-7000-1_10.
Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set. Measuring, processing, and merging many partial data sets of this kind requires new techniques which have since been implemented at several third-generation synchrotron facilities, and are described here. Among these, we particularly focus on the possibility of in situ measurements combined with in meso crystal preparations and data analysis with the XDS package and auxiliary programs.
受X射线自由电子激光测量方法进展的推动，在过去两年中人们认识到，从大分子标本晶体中收集数据的传统（或常规）方法可以通过对微晶进行同步加速器测量来补充，这些微晶单独而言不足以获得完整的数据集。测量、处理和合并许多这类部分数据集需要新技术，此后这些技术已在多个第三代同步加速器设施中得到应用，并在此进行描述。其中，我们特别关注原位测量与在介观晶体制备相结合的可能性，以及使用XDS软件包和辅助程序进行数据分析。
