Dauter Zbigniew
Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, IL, 60439, USA.
Methods Mol Biol. 2017;1607:165-184. doi: 10.1007/978-1-4939-7000-1_7.
Diffraction data acquisition is the final experimental stage of the crystal structure analysis. All subsequent steps involve mainly computer calculations. Optimally measured and accurate data make the structure solution and refinement easier and lead to more faithful interpretation of the final models. Here, the important factors in data collection from macromolecular crystals are discussed and strategies appropriate for various applications, such as molecular replacement, anomalous phasing, and atomic-resolution refinement are presented. Criteria useful for judging the diffraction data quality are also discussed.
衍射数据采集是晶体结构分析的最后一个实验阶段。所有后续步骤主要涉及计算机计算。经过优化测量且准确的数据会使结构解析和精修更加容易，并能对最终模型进行更可靠的阐释。在此，我们将讨论从大分子晶体收集数据时的重要因素，并介绍适用于各种应用（如分子置换、反常位相法和原子分辨率精修）的策略。我们还将讨论用于判断衍射数据质量的标准。
