验证化学物质对埃博拉病毒的灭活效果。

Validating the Inactivation Effectiveness of Chemicals on Ebola Virus.

作者信息

Haddock Elaine, Feldmann Friederike

机构信息

Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.

Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.

出版信息

Methods Mol Biol. 2017;1628:251-257. doi: 10.1007/978-1-4939-7116-9_20.

DOI:10.1007/978-1-4939-7116-9_20

PMID:28573626

Abstract

While viruses such as Ebola virus must be handled in high-containment laboratories, there remains the need to process virus-infected samples for downstream research testing. This processing often includes removal to lower containment areas and therefore requires assurance of complete viral inactivation within the sample before removal from high-containment. Here we describe methods for the removal of chemical reagents used in inactivation procedures, allowing for validation of the effectiveness of various inactivation protocols.

摘要

虽然像埃博拉病毒这样的病毒必须在高防护实验室中处理，但仍需要对病毒感染的样本进行处理，以便进行下游的研究测试。这种处理通常包括转移到较低防护区域，因此在从高防护区域移出之前，需要确保样本中的病毒完全失活。在此，我们描述了去除灭活程序中使用的化学试剂的方法，从而能够验证各种灭活方案的有效性。