伽马辐射作为一种有效灭活新兴病毒病原体的方法。
Gamma Irradiation as an Effective Method for Inactivation of Emerging Viral Pathogens.
机构信息
Division of Intramural Research (DIR), Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana.
Division of Intramural Research (DIR), Laboratory of Virology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana.
出版信息
Am J Trop Med Hyg. 2019 May;100(5):1275-1277. doi: 10.4269/ajtmh.18-0937.
Gamma irradiation using a cobalt-60 source is a commonly used method for the inactivation of infectious specimens to be handled safely in subsequent laboratory procedures. Here, we determined irradiation doses to safely inactivate liquid proteinaceous specimens harboring different emerging/reemerging viral pathogens known to cause neglected tropical and other diseases of regional or global public health importance. By using a representative arenavirus, bunyavirus, coronavirus, filovirus, flavivirus, orthomyxovirus, and paramyxovirus, we found that these enveloped viruses differed in their susceptibility to irradiation treatment with adsorbed doses for inactivation of a target dose of 1 × 10 50% tissue culture infectious dose (TCID)/mL ranging from 1 to 5 MRads. This finding seemed generally inversely correlated with genome size. Our data may help to guide other facilities in testing and verifying safe inactivation procedures.
利用钴-60 源进行伽马辐照是一种常用的方法，可用于灭活在随后的实验室程序中需要安全处理的传染性标本。在这里，我们确定了辐照剂量，以安全地灭活携带不同新兴/再现的病毒病原体的液体蛋白质标本，这些病毒病原体已知会导致被忽视的热带病和其他对区域或全球公共卫生具有重要意义的疾病。通过使用代表性的沙粒病毒、布尼亚病毒、冠状病毒、丝状病毒、黄病毒、正粘病毒和副粘病毒，我们发现这些包膜病毒对辐照处理的敏感性不同，吸附剂量为灭活目标剂量为 1×1050%组织培养感染剂量（TCID）/mL 的吸附剂量为 1 至 5 毫拉德。这一发现似乎与基因组大小呈负相关。我们的数据可能有助于指导其他设施进行测试和验证安全的失活程序。
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