Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Department of Plant Pathology, University of Georgia, Tifton, GA, USA.
J Antimicrob Chemother. 2017 Sep 1;72(9):2443-2446. doi: 10.1093/jac/dkx168.
Azole resistance in isolates of the fungus Aspergillus fumigatus has been associated with agricultural use of azole fungicides. Environmental isolation of resistant isolates has been reported in Asia, Africa, Europe and South America.
To determine whether A. fumigatus isolates containing TR34/L98H or TR46/Y121F/T289A can be found in fields in the USA treated with agricultural azoles.
Crop debris was collected and screened for A. fumigatus. All A. fumigatus isolates were screened for azole resistance. The CYP51A gene of azole-resistant isolates was sequenced. The population structure of a subset of isolates was determined using microsatellite typing.
This article identifies azole-resistant A. fumigatus isolates containing the TR34/L98H mutation in an experimental peanut field that had been treated with azole fungicides.
These findings suggest the development of resistance to azole antifungals in A. fumigatus may be present where agricultural azoles are used in the USA.
烟曲霉分离株中的唑类耐药性与农业用唑类杀真菌剂有关。已在亚洲、非洲、欧洲和南美洲报告了环境中分离出耐药分离株的情况。
确定在美国使用农业唑类处理过的田地中是否可以发现含有 TR34/L98H 或 TR46/Y121F/T289A 的烟曲霉分离株。
收集作物残体并筛选烟曲霉。对所有烟曲霉分离株进行唑类耐药性筛选。对唑类耐药分离株的 CYP51A 基因进行测序。使用微卫星分型确定部分分离株的种群结构。
本文在一个使用唑类杀真菌剂处理过的实验性花生田中鉴定出含有 TR34/L98H 突变的唑类耐药烟曲霉分离株。
这些发现表明，在美国使用农业唑类的情况下，烟曲霉对唑类抗真菌药物的耐药性可能已经出现。
