Zhu Qianqian, Li Na, Li Fang, Sang Jiao, Deng Huan, Han Qunying, Lv Yi, Li Chunyan, Liu Zhengwen
Department of Infectious Diseases, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China.
Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China; Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China.
Int Immunopharmacol. 2017 Aug;49:126-131. doi: 10.1016/j.intimp.2017.05.031. Epub 2017 May 30.
Lymphotoxin-β receptor (LTβR) signaling is involved in hepatitis B virus (HBV) infection, hepatitis and liver carcinogenesis. However, the potential association between LTBR polymorphisms and HBV infection remains unclear. This study investigated the associations between LTBR polymorphisms and chronic HBV infection and HBV-related hepatocellular carcinoma (HCC). The study included 409 patients with chronic HBV infection, 73 HBV infection resolvers, and 197 healthy controls. Two polymorphisms rs12354 and rs3759333 were selected and genotyped by polymerase chain reaction-ligase detection reaction method. The frequencies of rs12354 genotype GT and allele T in HBV infection resolvers were significantly higher than those in patients with chronic HBV infection and healthy controls (genotype GT: 38.4% vs. 22.2% and 38.4% vs. 20.8%, P=0.004 and P=0.004, respectively; allele T: 20.5% vs. 13.1% and 20.5% vs. 12.9%, P=0.017 and P=0.028, respectively). The frequencies of rs3759333 genotypes and alleles between HBV patients, HBV infection resolvers and healthy controls had no statistical difference. The genotype and allele frequencies of rs12354 and rs3759333 had no statistical differences between chronic hepatitis B and HBV-related HCC patients. The serum LTβR levels and the overall survival rate between HBV-related HCC patients carrying different rs12354 and rs3759333 genotypes had no statistical differences. These results suggest that the LTBR rs12354 polymorphism might be associated with the spontaneous resolution of HBV infection. Additional studies with large sample size are needed to confirm and extend these findings.
淋巴毒素-β受体（LTβR）信号传导参与乙型肝炎病毒（HBV）感染、肝炎及肝癌发生。然而，LTBR基因多态性与HBV感染之间的潜在关联仍不明确。本研究调查了LTBR基因多态性与慢性HBV感染及HBV相关肝细胞癌（HCC）之间的关联。研究纳入409例慢性HBV感染患者、73例HBV感染康复者及197例健康对照。选择两个多态性位点rs12354和rs3759333，并采用聚合酶链反应-连接检测反应法进行基因分型。HBV感染康复者中rs12354基因型GT及等位基因T的频率显著高于慢性HBV感染患者及健康对照（基因型GT：38.4%对22.2%及38.4%对20.8%，P分别为0.004和0.004；等位基因T：20.5%对13.1%及20.5%对12.9%，P分别为0.017和0.028）。HBV患者、HBV感染康复者及健康对照之间rs3759333基因型及等位基因频率无统计学差异。慢性乙型肝炎患者与HBV相关HCC患者之间rs12354和rs3759333的基因型及等位基因频率无统计学差异。携带不同rs12354和rs3759333基因型的HBV相关HCC患者的血清LTβR水平及总生存率无统计学差异。这些结果表明，LTBR rs12354多态性可能与HBV感染的自发清除有关。需要更多大样本研究来证实和扩展这些发现。
