Yu Hanwu, Huang Xiaobo, Yang Xiaoning, Liu Huibing, Zhang Meng, Zhang Xiangyu, Hang Ruiqiang, Tang Bin
Research Institute of Surface Engineering, Taiyuan University of Technology, No. 79 Yingze West Road, Taiyuan, China.
Research Institute of Surface Engineering, Taiyuan University of Technology, No. 79 Yingze West Road, Taiyuan, China.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:175-184. doi: 10.1016/j.msec.2017.04.063. Epub 2017 Apr 12.
It is acknowledged that ideal implant coatings should possess micro/nano-textured surface, have good interfacial bonding, and can release bioactive elements. In this study, we fabricated a Zn-incorporated micro/nano-textured surface by one-step high current anodization (HCA) in an aqueous solution with 10g/L of NaOH and different concentrations of Zn(NO) (4, 7, and 12g/L). The control group of Zn-free was fabricated in the electrolyte of 7g/L Zn(NO). Scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and inductively coupled plasma mass spectroscopy (ICP-MS) were used to analyze the morphology, composition, microstructure, and Zn release kinetics of the micro/nano-textured coatings. The biological properties of the surface structure were evaluated by cytotoxicity assay, cell viability, cytoskeletal assembly and alkaline phosphatase activity. Our results show the micro/nano-textured surface is composed of TiO mesoporous arrays, into which the Zn is demonstrated to be incorporated in the form of ZnO. The Zn content in the surface and release level of Zn can be tailored through varying Zn(NO) concentration in the electrolyte. In addition, the surface oxide layers show good interfacial bonding strength to the substrate. Compared with pure Ti and anodized Zn-free samples, the Zn-incorporated surface can upregulate osteoblast functions such as proliferation and alkaline phosphatase activity, which are assayed by MTT and ALP staining experiments, respectively. Collectively, this micro/nano-textured structure combined with high interfacial bonding strength and release of Zn render the material surface promising as orthopedic implant coatings.
人们公认，理想的植入物涂层应具有微/纳米纹理表面，具备良好的界面结合力，并能释放生物活性元素。在本研究中，我们通过在含有10g/L NaOH和不同浓度Zn(NO)（4、7和12g/L）的水溶液中进行一步高电流阳极氧化（HCA）制备了含锌的微/纳米纹理表面。不含锌的对照组是在7g/L Zn(NO)的电解液中制备的。使用扫描电子显微镜（SEM）、高分辨率透射电子显微镜（HR-TEM）、X射线光电子能谱（XPS）、X射线衍射（XRD）和电感耦合等离子体质谱（ICP-MS）来分析微/纳米纹理涂层的形态、成分、微观结构和锌释放动力学。通过细胞毒性试验、细胞活力、细胞骨架组装和碱性磷酸酶活性评估表面结构的生物学特性。我们的结果表明，微/纳米纹理表面由TiO中孔阵列组成，其中锌以ZnO的形式被证明掺入其中。表面锌含量和锌释放水平可通过改变电解液中Zn(NO)的浓度来调整。此外，表面氧化层与基底显示出良好的界面结合强度。与纯钛和阳极氧化的无锌样品相比，含锌表面可上调成骨细胞功能，如增殖和碱性磷酸酶活性，分别通过MTT和ALP染色实验进行测定。总的来说，这种微/纳米纹理结构与高界面结合强度和锌释放相结合，使该材料表面有望成为骨科植入物涂层。
