Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, PR China.
Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Nov 1;80:411-416. doi: 10.1016/j.msec.2017.06.008. Epub 2017 Jun 16.
Fluorescent polymeric nanoparticles (FPNs) with aggregation-induced emission (AIE) characteristics have attracted much attention for biomedical applications due to their remarkable AIE feature, high water dispersity and desirable biocompatibility. The development of facile and effective strategies for fabrication of these AIE-active FPNs therefore should be of great importance for their biomedical applications. In this work, we reported that a catalyst-free thiol-yne click reaction can be utilized for fabrication of AIE-active FPNs in short reaction time and even without protection of inert gas. The results indicated that the obtained AIE-active amphiphilic copolymers (PEGMA-PhE) can readily self-assemble into luminescent nanoparticles (PEGMA-PhE FPNs) with high water dispersity, uniform size and morphology, red fluorescence. Cell viability examination and cell uptake behavior of PEGMA-PhE FPNs confirmed that these AIE-active FPNs possess low toxicity towards cells and can be easily internalized by cells through non-specific route. Therefore the remarkable properties of PEGMA-PhE FPNs such as high water dispersity, AIE-active fluorescence and nanoscale size as well as excellent biocompatibility make them promising for biomedical applications.
具有聚集诱导发光（AIE）特性的荧光聚合物纳米粒子（FPNs）由于其显著的 AIE 特性、高水分散性和理想的生物相容性，引起了人们对生物医学应用的极大关注。因此，开发简便有效的制备这些 AIE 活性 FPNs 的策略对于它们的生物医学应用应该非常重要。在这项工作中，我们报告说，无催化剂的硫醇-炔点击反应可用于在短反应时间内甚至在没有惰性气体保护的情况下制备 AIE 活性 FPNs。结果表明，所得的 AIE 活性两亲性共聚物（PEGMA-PhE）可以很容易地自组装成具有高水分散性、均匀尺寸和形态、红色荧光的发光纳米粒子（PEGMA-PhE FPNs）。PEGMA-PhE FPNs 的细胞活力检测和细胞摄取行为证实，这些 AIE 活性 FPNs 对细胞的毒性较低，并且可以通过非特异性途径很容易地被细胞内化。因此，PEGMA-PhE FPNs 的显著性质，如高水分散性、AIE 活性荧光和纳米级尺寸以及优异的生物相容性，使它们有望用于生物医学应用。
