一种用于高灵敏度检测锌（II）的仿生荧光化学传感器及其在细胞成像中的应用。
A biomimetic fluorescent chemosensor for highly sensitive zinc(ii) detection and its application for cell imaging.
作者信息
Yan Rui, Wang Zhi, Du Zongliang, Wang Haibo, Cheng Xu, Xiong Junjie
机构信息
Textile Institute, College of Light Industry, Textile and Food Engineering, Sichuan University Chengdu 610065 China
State Key Laboratory of Biotherapy, Sichuan University Chengdu 610041 China.
出版信息
RSC Adv. 2018 Sep 26;8(58):33361-33367. doi: 10.1039/c8ra06501b. eCollection 2018 Sep 24.
To fabricate a novel biomimetic fluorescent chemosensor, PSaAEMA--PMPC was synthesized atom transfer radical polymerization, and this copolymer could be used for the detection of zinc(ii) and cell imaging. A series tests with various metal ions verified the specific fluorescence response behavior. This novel biomimetic fluorescent chemosensor exhibits excellent selectivity for Zn ions over a wide range of tested metal ions in an aqueous solution. Moreover, cytotoxicity and bio-imaging tests were conducted to study the potential bio-application of the chemosensor. Owing to the biomimetic portion (phosphorylcholine), this copolymer possesses outstanding biocompatibility and could clearly image cells. The results indicated that PSaAEMA--PMPC has great potential for application in zinc(ii) detection and cell imaging.
为制备一种新型的仿生荧光化学传感器，通过原子转移自由基聚合反应合成了聚（苯乙烯-alt-丙烯酸乙酯）-b-聚（2-甲氧基-2-丙基丙烯酸酯）（PSaAEMA--PMPC），该共聚物可用于锌（II）的检测和细胞成像。一系列针对各种金属离子的测试验证了其特定的荧光响应行为。这种新型的仿生荧光化学传感器在水溶液中对多种测试金属离子表现出对锌离子的优异选择性。此外，还进行了细胞毒性和生物成像测试以研究该化学传感器的潜在生物应用。由于其仿生部分（磷酰胆碱），该共聚物具有出色的生物相容性并且能够清晰地对细胞成像。结果表明，PSaAEMA--PMPC在锌（II）检测和细胞成像方面具有巨大的应用潜力。
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