Leverve X M, Groen A K, Verhoeven A J, Tager J M
FEBS Lett. 1985 Feb 11;181(1):43-6. doi: 10.1016/0014-5793(85)81110-8.
Isolated hepatocytes from fasted rats were perifused with glycerol as gluconeogenic substrate. Stimulation of gluconeogenesis with phenylephrine (10(-5) M) as alpha-adrenergic agonist consisted of two distinct phases. The first phase was a transient stimulation of gluconeogenesis and was accompanied by transient changes in cytosolic and mitochondrial redox state; this phase was abolished by the transaminase inhibitor aminooxyacetate. The second phase was a stable stimulation of less magnitude, without change in redox state and insensitive to addition of aminooxyacetate. It is concluded that the first phase is due to a transient enhancement of flux through the malate/aspartate shuttle and that the stable phase is probably due to a stimulation of mitochondrial glycerol-3-phosphate dehydrogenase and glycerol kinase.
将禁食大鼠的分离肝细胞用甘油作为糖异生底物进行灌流。用苯肾上腺素（10⁻⁵ M）作为α-肾上腺素能激动剂刺激糖异生包括两个不同阶段。第一阶段是糖异生的短暂刺激，并伴有胞质和线粒体氧化还原状态的短暂变化；该阶段被转氨酶抑制剂氨氧乙酸消除。第二阶段是幅度较小的稳定刺激，氧化还原状态无变化，且对氨氧乙酸的添加不敏感。得出的结论是，第一阶段是由于通过苹果酸/天冬氨酸穿梭的通量短暂增强，而稳定阶段可能是由于线粒体甘油-3-磷酸脱氢酶和甘油激酶的刺激。
