Department of Advanced Zoology and Biotechnology, Guru Nanak College, Chennai, Tamil Nadu, 600042, India.
Environ Sci Pollut Res Int. 2017 Jul;24(20):16927-16932. doi: 10.1007/s11356-017-9196-x. Epub 2017 Jun 2.
The biological quality of secondary treated sewage effluent was evaluated using a toolbox approach, which combined a larval developmental bioassay and measurement of fecal indicator organisms. The zoea developmental toxicity of Scylla serrata from stage I to stage II was determined by exposing to a range of secondary treated sewage concentrations. Results indicated that the relative progress of zoea stage I to zoea stage II negatively correlated with increasing sewage concentrations. Data was analyzed statistically to determine lethal, median lethal, sublethal, low observed effect, and no observed effect concentrations. Water samples collected along the Buckingham canal discharge zone were also analyzed for its toxicity to the larval development. Fecal indicator organisms chosen to determine the water quality were E. coli, enterococci, C. perfrigens, and F+ coliphages. Concentrations of these fecal markers were determined in the raw influent, primary treated effluent, secondary treated effluent, and in four discharge zone sites. Data showed that this biological toolbox is helpful for providing baseline information on the effectiveness of the wastewater treatment and environmental health of the discharge zone.
采用工具盒方法评估二级处理污水的生物质量，该方法结合了幼虫发育生物测定和粪便指示生物的测量。通过暴露于一系列二级处理污水浓度，确定了从第一期到第二期的锯缘青蟹幼虫的发育毒性。结果表明，幼虫第一期到幼虫第二期的相对进展与污水浓度的增加呈负相关。对数据进行了统计学分析，以确定致死、半数致死、亚致死、低观察效应和无观察效应浓度。还对沿白金汉运河排放区采集的水样进行了毒性分析，以评估其对幼虫发育的影响。选择粪便指示生物来确定水质的有大肠杆菌、肠球菌、C. perfrigens 和 F+噬菌体。在原进水、一级处理出水、二级处理出水中以及在四个排放区现场测定了这些粪便标记物的浓度。数据表明，该生物工具盒有助于提供有关废水处理效果和排放区环境健康的基线信息。
