Universidade Federal Rural de Pernambuco-UFRPE, Recife, PE, Brazil.
Universidade Regional do Cariri-URCA, Crato, CE, Brazil.
Biomed Pharmacother. 2017 Aug;92:554-561. doi: 10.1016/j.biopha.2017.05.098.
The increase in microorganisms with resistance to medications has caused a strong preoccupation within the medical and scientific community. Animal toxins studies, such as parotoid glandular secretions from amphibians, possesses a great potential in the development of drugs, such as antimicrobials, as these possess bioactive compounds. It was evaluated Rhinella jimi (Stevaux, 2002) glandular secretions against standard and multi-resistant bacterial strains; the effect of secretions combined with drugs; and determined the toxicity using two biologic in vivo models, and a in vitro model with mice livers. Standard strains were used for the determination of the Minimum Inhibitory Concentration (MIC), while for the modulatory activity of antibiotics, the clinical isolates Escherichia coli 06, Pseudomonas aeruginosa 03 and Staphylococcus aureus 10 were used. Modulatory activity was evaluated by the broth microdilution method with aminoglycosides and β-lactams as target antibiotics. The secretions in association with the antibiotics have a significant reduction in MIC, both the aminoglycosides and β-lactams. The toxicity and cytotoxicity results were lower than the values used in the modulation. R. jimi glandular secretions demonstrated clinically relevant results regarding the modulation of the tested antimicrobials.
微生物对抗药物的耐药性增加引起了医学界和科学界的强烈关注。动物毒素研究，如来自两栖动物的腮腺腺分泌物，在开发药物方面具有巨大潜力，因为这些分泌物含有生物活性化合物。本研究评估了 Rhinella jimi（Stevaux，2002）的腺体分泌物对标准和多耐药菌的抑制作用；研究了分泌物与药物联合使用的效果；并使用两种体内生物模型和一种小鼠肝脏的体外模型来确定毒性。标准菌株用于测定最小抑菌浓度（MIC），而对于抗生素的调节活性，使用临床分离株大肠杆菌 06、铜绿假单胞菌 03 和金黄色葡萄球菌 10。通过肉汤微量稀释法评估氨基糖苷类和β-内酰胺类作为目标抗生素的调节活性。抗生素联合使用后，氨基糖苷类和β-内酰胺类的 MIC 显著降低。毒性和细胞毒性结果均低于调节作用中使用的值。R. jimi 腺体分泌物在调节测试的抗菌药物方面显示出具有临床相关性的结果。
