石墨烯纳米粒子作为诱导成骨和骨传导的干细胞和骨再生平台。
Graphene nanoparticles as osteoinductive and osteoconductive platform for stem cell and bone regeneration.
机构信息
Tissue Regeneration Laboratory, Department of Large Animal Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA; Department of Surgery, Faculty of Veterinary Medicine, Alexandria University, Egypt.
Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, Arkansas, USA.
出版信息
Nanomedicine. 2017 Oct;13(7):2117-2126. doi: 10.1016/j.nano.2017.05.009. Epub 2017 Jun 1.
The potential of graphene-based nanoparticles (GNPs) has recently gained significant attention in biomedicine, especially in tissue engineering. In this study, we investigated the osteoinductive and osteoconductive effects of low oxygen content graphene (LOG) nanoparticles on adult mesenchymal stem cells (MSCs) in vitro and in vivo. We showed that adult goat MSCs were viable in the presence of 0.1 mg/mL LOG and retained their stem cell properties. A 3D scaffold made from agarose was used to encapsulate MSCs and LOG nanoparticles. Scanning electron microscopy demonstrated the cell morphology and adherence of MSCs to LOG in the 3D form. The LOG and MSCs in the 3D scaffold were xenogenically implanted into a rat unicortical tibial bone defect. The combination of MSCs and LOG nanoparticles resulted in improved active bone formation and increased mineralization. These results strengthen the applicability of LOG nanoparticles as an adjunct treatment for bone tissue engineering.
基于石墨烯的纳米粒子（GNPs）在生物医药领域，特别是在组织工程学中，具有很大的应用潜力。在这项研究中，我们研究了低氧含量石墨烯（LOG）纳米粒子对成体间充质干细胞（MSCs）的体外和体内成骨诱导和骨传导作用。结果表明，在 0.1mg/ml LOG 存在的情况下，成体山羊 MSCs 仍然具有活力，并保持其干细胞特性。使用琼脂糖制成的 3D 支架来包封 MSC 和 LOG 纳米粒子。扫描电子显微镜显示了 MSC 在 3D 形态下的细胞形态和对 LOG 的黏附。LOG 和 MSC 在 3D 支架中的混合物被异种植入大鼠单皮质胫骨骨缺损中。MSC 和 LOG 纳米粒子的组合导致了活跃骨形成和矿化的增加。这些结果增强了 LOG 纳米粒子作为骨组织工程辅助治疗的适用性。
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