Shoba Ekambaram, Lakra Rachita, Syamala Kiran Manikantan, Korrapati Purna Sai
Biological Materials Laboratory, CSIR-Central Leather Research Institute, Chennai, 600 020, India.
Biomed Mater. 2017 Jun 5;12(3):035005. doi: 10.1088/1748-605X/aa6684.
The physiological and pathological complexity of the wound healing process makes it more challenging to design an ideal tissue regeneration scaffold. Precise scaffolding with high drug loading efficiency, efficient intracellular efficacy for therapeutic delivery, minimal nonspecific cellular and blood protein binding, and maximum biocompatibility forms the basis for an ideal delivery system. This paper describes a combinational multiphasic delivery system, where biomolecules are delivered through the fabrication of coaxial electrospinning of different biocompatible polymers. The ratio and specificity of polymers for specific biofunction are optimized and the delivery system is completely characterized with reference to the mechanical property and structural integrity of bromelain (debridement enzyme) and salvianolic acid B (pro-angiogenesis and re-epithelialization). The in vitro release profile illustrated the sustained release of debriding protease and bioactive component in a timely fashion. The fabricated scaffold showed angiogenic potential through in vitro migration of endothelial cells and increased new capillaries from the existing blood vessel in response to an in ovo chicken chorioallantoic membrane assay. In addition, in vivo studies confirm the efficacy of the fabricated scaffold. Our results therefore open up a new avenue for designing a bioactive combinational multiphasic delivery system to enhance wound healing.
伤口愈合过程的生理和病理复杂性使得设计理想的组织再生支架更具挑战性。具有高药物负载效率、高效细胞内治疗递送效果、最小非特异性细胞和血液蛋白结合以及最大生物相容性的精确支架构成了理想递送系统的基础。本文描述了一种组合多相递送系统，其中生物分子通过不同生物相容性聚合物的同轴电纺丝制造来递送。针对特定生物功能的聚合物比例和特异性得到优化，并且参照菠萝蛋白酶（清创酶）和丹酚酸B（促血管生成和再上皮化）的机械性能和结构完整性对递送系统进行了全面表征。体外释放曲线表明清创蛋白酶和生物活性成分能及时持续释放。通过体外内皮细胞迁移以及在鸡胚绒毛尿囊膜试验中对现有血管新毛细血管生成增加的观察，所制备的支架显示出血管生成潜力。此外，体内研究证实了所制备支架的功效。因此，我们的结果为设计生物活性组合多相递送系统以促进伤口愈合开辟了一条新途径。
