Liu Chunxiu, Cai Haoyuan, Jia Jian, Cao Tianyang, Xu Chenghua, Liu Chang
Technol Health Care. 2017 Jul 20;25(S1):151-156. doi: 10.3233/THC-171317.
C-reactive protein (CRP) is used to evaluate the evolution of infections and sepsis in critically ill patients. For POCT testing, biosensor-based detection techniques offer quick and convenient application.
A prototype three dimensional chip was fabricated based on a new optomagnetic method to achieve the rapid detection of CRP.
This work investigates a new technology for the quick quantitative detection of the C-reactive protein (CRP) by total internal reflection magnetic imaging (TIRMI) on a three dimensional optomagnetic sensor. Transparent glass and hydrophilic plastic film with channels were used to construct the three dimensional sensor. The magnetic nanoparticles and immunological reagent were immobilized on the reaction area of the sensor. Samples were detected using total internal reflection magnetic spot imaging (TIRMI) based on a sandwich magnetic immunoassay by one-step assay.
The developed 3D biosensor-TIRMI method showed a wide dynamic linear range (0.2-200 ng/ml) and quick detection (5 min) with low-sample volume (10 μL).
We have presented a three dimensional optical protein chip that fulfills the demanding for point-of-care diagnostics in terms of ease-of-use (one step assay), miniaturization, assay time. This approach shows great promise for application in clinical investigations of biological samples.
C反应蛋白（CRP）用于评估重症患者感染和脓毒症的进展情况。对于即时检验（POCT）检测，基于生物传感器的检测技术应用快速便捷。
基于一种新型光磁方法制备三维芯片，以实现对CRP的快速检测。
本研究探讨了一种通过三维光磁传感器上的全内反射磁成像（TIRMI）对C反应蛋白（CRP）进行快速定量检测的新技术。使用透明玻璃和亲水塑料膜构建带通道的三维传感器。将磁性纳米颗粒和免疫试剂固定在传感器的反应区域。基于夹心磁免疫分析法，通过一步法使用全内反射磁斑点成像（TIRMI）对样品进行检测。
所开发的三维生物传感器 - TIRMI方法显示出较宽的动态线性范围（0.2 - 200 ng/ml），检测速度快（5分钟），且样品用量少（10μL）。
我们展示了一种三维光学蛋白质芯片，该芯片在易用性（一步法检测）、小型化和检测时间方面满足了即时诊断的要求。这种方法在生物样品的临床研究中具有很大的应用前景。
