Department of Molecular Biology, Princeton University, New Jersey 08544-1014
Lewis-Sigler Institute for Integrative Genomics, Princeton University, New Jersey 08544-1014
G3 (Bethesda). 2017 Jul 5;7(7):2219-2226. doi: 10.1534/g3.117.040022.
The yeast has emerged as a superior model organism. Selection of distinct laboratory strains of with unique phenotypic properties, such as superior mating or sporulation efficiencies, has facilitated advancements in research. W303 is one such laboratory strain that is closely related to the first completely sequenced yeast strain, S288C. In this work, we provide a high-quality, annotated genome sequence for W303 for utilization in comparative analyses and genome-wide studies. Approximately 9500 variations exist between S288C and W303, affecting the protein sequences of ∼700 genes. A listing of the polymorphisms and divergent genes is provided for researchers interested in identifying the genetic basis for phenotypic differences between W303 and S288C. Several divergent functional gene families were identified, including flocculation and sporulation genes, likely representing selection for desirable laboratory phenotypes. Interestingly, remnants of ancestor wine strains were found on several chromosomes. Finally, as a test of the utility of the high-quality reference genome, variant mapping revealed more accurate identification of accumulated mutations in passaged mismatch repair-defective strains.
酵母已成为一种优越的模式生物。选择具有独特表型特性的不同实验室菌株，例如优良的交配或孢子形成效率，促进了研究的进展。W303 就是这样一种实验室菌株，它与第一个完全测序的酵母菌株 S288C 密切相关。在这项工作中，我们提供了 W303 的高质量、注释基因组序列，以供比较分析和全基因组研究使用。S288C 和 W303 之间存在大约 9500 个差异，影响了约 700 个基因的蛋白质序列。对于那些有兴趣确定 W303 和 S288C 之间表型差异的遗传基础的研究人员，我们提供了一份多态性和分歧基因的清单。确定了几个分歧的功能基因家族，包括絮凝和孢子形成基因，可能代表了对理想实验室表型的选择。有趣的是，在几个染色体上发现了几个祖先葡萄酒菌株的残余物。最后，作为对高质量参考基因组实用性的测试，变异映射揭示了在经过传代的错配修复缺陷菌株中积累突变的更准确识别。
