Sairkar Pramod Kumar, Sharma Anjana, Shukla N P
Centre of Excellence in Biotechnology, M. P. Council of Science and Technology, Bhopal, Madhya Pradesh, India.
Department of Postgraduate Studies and Research in Biological Science, Bacteriology Laboratory, Rani Durgavati University, Jabalpur, Madhya Pradesh, India.
J Pharm Bioallied Sci. 2017 Jan-Mar;9(1):1-7. doi: 10.4103/0975-7406.206225.
Guggulsterone (GS) is a plant steroid and bioactive compound present in gum Guggul of . An Indian herbal medicine system "Ayurveda" has a long history of use of gum Guggul and plant extract of as medicine for the treatment of various illnesses. Complex nature, low availability, and inconsistency of phytoconstituents make its analysis of difficult tasks.
In this work, six different Guggul-based herbal formulations were examined for estimation of GS and their isomers (E and Z) through high-performance thin-layer chromatography technique.
For that various concentrations of standard E-GS and Z-GS (50 ng-250 ng/spot) with samples (20 μg/spot) were applied on silica gel coated aluminum plate and developed with the mobile phase of toluene: ethyl acetate: formic acid: methanol (6:2:1:0.5). The scanning was performed at 254 nm wavelength and the absorbance (scan) spectrum of E-GS and Z-GS peak was generated at 200 nm-400 nm wavelength range.
R value and scan spectrum pattern of the samples reveal that they contain either one form of GS (E-GS, Z-GS) or both. The quantity of E-GS and Z-GS within the samples was ranged from 0.230 ± 0.0040-0.926 ± 0.0168% to 0.537 ± 0.0026-0.723 ± 0.0177%, respectively.
古古甾酮（GS）是一种植物甾体和生物活性化合物，存在于古古胶中。印度草药医学体系“阿育吠陀”使用古古胶及其植物提取物治疗各种疾病已有悠久历史。其成分复杂、可得性低且植物成分不一致，使得对其进行分析成为一项艰巨任务。
在本研究中，通过高效薄层色谱技术对六种不同的基于古古胶的草药制剂进行检测，以测定GS及其异构体（E型和Z型）的含量。
为此，将不同浓度的标准E - GS和Z - GS（50 ng - 250 ng/点）与样品（20 μg/点）点于硅胶涂层铝板上，用甲苯：乙酸乙酯：甲酸：甲醇（6:2:1:0.5）的流动相展开。在254 nm波长处进行扫描，并在200 nm - 400 nm波长范围内生成E - GS和Z - GS峰的吸光度（扫描）光谱。
样品的Rf值和扫描光谱图表明，它们要么含有一种形式的GS（E - GS、Z - GS），要么同时含有两种。样品中E - GS和Z - GS的含量分别在0.230±0.0040 - 0.926±0.0168%至0.537±0.0026 - 0.723±0.0177%之间。
