Shim Eugene, Song Eunju, Choi Kyoung Sook, Choi Hyuk-Joon, Hwang Jinah
Department of Food and Nutrition, Soongeui Women's College, Seoul 04628, Korea.
Department of Food and Nutrition, College of Natural Sciences, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin, Gyeonggi 17058, Korea.
Nutr Res Pract. 2017 Jun;11(3):173-179. doi: 10.4162/nrp.2017.11.3.173. Epub 2017 Apr 10.
BACKGROUND/OBJECTIVES: Blume (GEB), a traditional herbal medicine, has been used to treat a wide range of neurological disorders (, paralysis and stroke) and skin problems (, atopic dermatitis and eczema) in oriental medicine. This study was designed to investigate whether GEB extract inhibits melanogenesis activity in murine B16F10 melanoma.
MATERIALS/METHOD: Murine B16F10 cells were treated with 0-5 mg/mL of GEB extract or 400 µg/mL arbutin (a positive control) for 72 h after treatment with/without 200 nM alpha-melanocyte stimulating hormone (α-MSH) for 24 h. Melanin concentration, tyrosinase activity, mRNA levels, and protein expression of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein ()1, and were analyzed in α-MSH-untreated and α-MSH-treated B16F10 cells.
Treatment with 200 nM α-MSH induced almost 2-fold melanin synthesis and tyrosinase activity along with increased mRNA levels and protein expression of MITF, tyrosinase, and . Irrespective of α-MSH stimulation, GEB extract at doses of 0.5-5 mg/mL inhibited all these markers for skin whitening in a dose-dependent manner. While lower doses (0.5-1 mg/mL) of GEB extract generally had a tendency to decrease melanogenesis, tyrosinase activity, and mRNA levels and protein expression of MITF, tyrosinase, , and , higher doses (2-5 mg/mL) significantly inhibited all these markers in α-MSH-treated B16F10 cells in a dose-dependent manner. These inhibitory effects of the GEB extract at higher concentrations were similar to those of 400 µg/mL arbutin, a well-known depigmenting agent.
These results suggest that GEB displays dose-dependent inhibition of melanin synthesis through the suppression of tyrosinase activity as well as molecular levels of MITF, tyrosinase, , and in murine B16F10 melanoma. Therefore, GEB may be an effective and natural skin-whitening agent for application in the cosmetic industry.
背景/目的：Blume（GEB）是一种传统草药，在东方医学中已被用于治疗多种神经系统疾病（如瘫痪和中风）以及皮肤问题（如特应性皮炎和湿疹）。本研究旨在调查GEB提取物是否能抑制小鼠B16F10黑色素瘤细胞中的黑色素生成活性。
材料/方法：在用/不用200 nMα-黑素细胞刺激素（α-MSH）处理24小时后，用0 - 5 mg/mL的GEB提取物或400 μg/mL熊果苷（阳性对照）处理小鼠B16F10细胞72小时。分析未用α-MSH处理和用α-MSH处理的B16F10细胞中的黑色素浓度、酪氨酸酶活性、mRNA水平以及小眼畸形相关转录因子（MITF）、酪氨酸酶、酪氨酸酶相关蛋白1和2的蛋白表达。
用200 nMα-MSH处理可诱导黑色素合成和酪氨酸酶活性增加近2倍，同时MITF、酪氨酸酶、1和2的mRNA水平及蛋白表达也增加。无论α-MSH刺激与否，0.5 - 5 mg/mL剂量的GEB提取物均以剂量依赖方式抑制所有这些皮肤美白标志物。虽然较低剂量（0.5 - 1 mg/mL）的GEB提取物通常有降低黑色素生成、酪氨酸酶活性以及MITF、酪氨酸酶、1和2的mRNA水平及蛋白表达的趋势，但较高剂量（2 - 5 mg/mL）能以剂量依赖方式显著抑制α-MSH处理的B16F10细胞中的所有这些标志物。GEB提取物在较高浓度下的这些抑制作用与400 μg/mL熊果苷（一种知名的色素脱除剂）的作用相似。
这些结果表明，GEB通过抑制酪氨酸酶活性以及小鼠B16F10黑色素瘤细胞中MITF、酪氨酸酶、1和2的分子水平，呈现出剂量依赖性的黑色素合成抑制作用。因此，GEB可能是一种可应用于化妆品行业的有效且天然的皮肤美白剂。
