Taborda Carlos P, Travassos Luiz R
Institute of Biomedical Sciences, Department of Microbiology, University of São Paulo, São Paulo, Brazil.
Laboratory of Medical Mycology IMTSP/LIM53/HCFMUSP, University of São Paulo, São Paulo, Brazil.
Methods Mol Biol. 2017;1625:113-128. doi: 10.1007/978-1-4939-7104-6_9.
The chapter reviews methods utilized for the isolation and characterization of a promising immunogen candidate, aiming at a human vaccine against paracoccidioidomycosis. Peptide P10 carries a T-CD4+ epitope and was identified as an internal sequence of the major diagnostic antigen known as gp43 glycoprotein. It successfully treated massive intratracheal infections by virulent Paracoccidioides brasiliensis in combination with chemotherapy.An introduction about the systemic mycosis was found essential to understand the various options that were considered to design prophylactic and therapeutic vaccine protocols using peptide P10.
本章回顾了用于分离和鉴定一种有前景的免疫原候选物的方法，目标是研发一种针对副球孢子菌病的人类疫苗。肽P10带有一个T-CD4+表位，被鉴定为主要诊断抗原即gp43糖蛋白的内部序列。它与化疗联合成功治疗了巴西副球孢子菌引起的大规模气管内感染。发现有必要先介绍一下全身性真菌病，以便理解为设计使用肽P10的预防性和治疗性疫苗方案而考虑的各种选择。
